Clinical diagnosis is essential for the identification of infectious diseases like malaria, hepatitis, etc. Effective clinical diagnosis depends on the experience of the clinician as well as the accuracy, sensitivity and specificity of the diagnostic tools and protocols used. In case of biosensors, which are devices that couple biological sensing elements to transducers to generate a signal in response to a specific analyte, such qualities, especially specificity, may be achieved with suitable design.
The rapid progress in the microfabrication technologies, driven by demand for smaller and cheaper electronics, has aided in the advancement of technologies to miniaturize sensors as well, particularly since some of the microfabrication methods have been adopted for use with polymers and other materials besides silicon and its derivatives. This research describes one such configuration which may be suitably adapted for use in sensing biological molecules. The objective of this work was to design and develop a label-free optical biosensor based on the principles of Guided Mode Resonance Filters (GMRF). Although some effort has been made towards development of such sensors elsewhere, they rely on expensive material like quartz as a substrate. This study was aimed at establishing new, cost effective sensing schemes to detect antigen-antibody interaction on the surface of the sensor (i.e. develop a GMRF based immunosensor). The research includes the selection of the material, fabrication of the nano dimensional optical GMRF device, surface modification, protein immobilization, theoretical analysis and characterization of the fabricated biosensor for detection.
Gajanan D. Nagare, received his Bachelor, Master degree in Instrumentation & Control from North Maharashtra University, Jalgaon & Government College of Engineering, Pune in 2000 & 2013 respectively. He Joined IIT Bombay in 2004 for his PhD research on “Guided Mode Resonance Filter Based Optical Biosensors”. He also worked as a Student Lab-in-Charge for MEMS laboratory & Centre of Excellence in Nano-electronics. He was also involved in the IEEE activities at IIT Bombay. It involves site preparation for new instruments, installation of newly arrived instruments, maintaining clean room discipline in the lab, updating the policies for the lab users (internal and external), training newcomers in the laboratory, maintaining equipment and arranging for repairs and managing the man power to accomplish tasks. In 2009, He worked as Project associate for Indian Nano-electronics User’s Programme (INUP) at CEN, IIT Bombay. It involved process development and scheduling fabrication processes for the completion of time bounded research in different fields. He also worked as Post-Doctoral Fellow at University of Alberta, Canada in 2010. During his PDF, he worked on Development of Biosensor for the detection of Lysteria in collaboration with Industry, AQL Inc., Canada. He applied for the patent at University Alberta, Canada along with Industry partner.
He joined Vidyalankar Institute of Technology in 2011 as Associate professor. He then promoted as a Professor & Head of the Department of Biomedical Engineering from 2013. His primary work includes teaching UG & PG students and R & D activities at VIT Mumbai. He has active collaboration with Department of Biomedical Engineering, IIT Bombay. He is research project reviewer by Manipal University, Dubai Campus, UAE, January 2014. He is Board of studies Member for BCUD Solapur University, February 2015. He is involved in Syllabus setting of Biomedical Microsystems for Mumbai University as Chairperson of the committee, 2014. He is reviewer for Biomedical Engineering Research (BER), Nanoletters, Springer publication, Journal of Material Science and IJBCE. Nominated as “Active Researcher” in nanoscience and technology by Department of Science and Technology, Govt. of India, November 2012. He is a Life time membership of ISTE (LM 80988), Biomedical Engineering Society of India- LM1079, International Association of Advanced Materials-78961911372 & Red Cross society.