Poster Presentations

Poster Presentations

Abstract

Service from the military special forces appointments participating in combat operations, as a rule, takes place in the extreme situation, the deterministic impact of a large variety of factors of different nature: From the socio-psychological to the natural-climatic and physical. Complex Psychogenic and physical factors adversely affect the mental Status, the state of physiological functions and professional the efficiency of military personnel .Therefore, it is important to determine the number of people in this contingent stress resistance - adequate response of a person to non-standard Situation. In most studies, the definition of stress resistance is produced by the methods of psychological testing However, their known subjectivity is not allows you to specifically and qualitatively characterize the desired ability. As objective methods are currently used variability of the heart rhythm. But the ideology of this method is based on traditional ideas about the corrective effect of the autonomic nervous system on a heart rhythm. The latter circumstance reduces the diagnostic the value of the method (interpretation of the results). V.M. Pokrovsky put forward the concept of hierarchical Heart rhythmogenesis organization (2007) Based on this concept, VM. Pokrovsky (2010) was developed a test of cardiopulmonary synchronism, which is objective, Integratively allows to assess the regulatory-adaptive status. By Dynamics of the regulatory-adaptive status in the initial state and at effects of the stress factor can assess the level of stress resistance person. The level of stress resistance of servicemen of special appointment and its dependence on the experience of the service in the zones of special risk not studied . The purpose of the work is to assess the stress-resistance of servicemen special purpose and its dependence on the experience of serving in the areas of special risk .

Biography

Speaker
Viktor Glebov / RUDN
Russian Federation

Abstract

Efavirenz, a BCS class II drug, is insoluble in water but freely soluble in acetone and in alcohol. It belongs to BCS class II category with high lipophilicity (log P=5.4), poor aqueous solubility (4 μg/mL), low intrinsic dissolution rate (0.037 mg/cm2/min), limited oral bioavailability (40–50%), and high intersubject variability. In the present study, efavirenz and β-Cyclodextrin (β-CD) solid dispersions were prepared with a view to study the effect and influence of β-CD on the solubility and dissolution rate of this poorly aqueous soluble drug. Phase solubility profile revealed that the solubility of efavirenz was increased in the presence of β-CD and was classified as AL-type. Effect of variable, such as drug:carrier ratio, was studied. Physical characterization of the solid dispersion was characterized by Fourier transform infrared spectroscopy (FT-IR) and Differential scanning calorimetry (DSC). These studies revealed that a distinct loss of drug crystallinity in the solid molecular dispersions is ostensibly accounting for enhancement of dissolution rate in distilled water. The drug release from the prepared solid dispersion exhibited a first order kinetics. Solid dispersions of efavirenz showed a 4.06 times increase in dissolution rate over the pure drug.

Biography

Dr.D.Nagasamy Venkatesh has completed his Ph.D from The Tamil Nadu Dr.MGR Medical University, Chennai, Tamil Nadu in 2011 under the Faculty of Pharmacy. Presently, He is working as Assistant Professor at JSS College of Pharmacy, Ooty. Tamil Nadu. He has published more than 45 papers in reputed journals and has been serving as an editorial board member of peer reviewed international and national journal.

Speaker
D.Nagasamy Venkatesh / JSS college of Pharmacy
India

Abstract

The goal of the work described in this abstract was to evaluate a new, small, material-sparing freeze dryer, denoted the “Mini-Freeze Dryer or Mini-FD”, capable of reproducing the product temperature history of larger freeze dryers, thereby facilitating scale-up. The Mini-FD wall temperatures can be controlled to mimic loading procedures and dryer process characteristics of larger dryers. The Mini-FDis equipped with a Tunable Diode Laser Absorption Spectroscopy (TDLAS) water vapor mass flow monitor and with other advanced process analytical technology (PAT) sensors. Drying experiments were performed to demonstrate scalability to larger freeze dryers, including the determination of vial heat transfer coefficients, Kv. Product temperature histories during Kv runs were evaluated and compared with those obtained with a commercial laboratory scale freeze dryer (LyoStar II) for sucrose and mannitol product formulations. When the Mini-FD wall temperature was set at the LyoStar II band temperature (-20°C) to mimic lab dryer edge vials, edge vials drying in the Mini-FDpossessedan average Kvwithin 5%of those obtained during drying in the LyoStar II. When the wall temperatureofthe Mini-FD was set equal to the central vial product temperature,edge vials behaved as center vials, possessing a Kv value within 5% of those measured in the LyoStar II. During both Kvruns and complete product freeze drying runs, the temperature-time profiles for the average edge vials and central vial in the Mini-FD agreed well with the average edge and average central vials of the LyoStar II.

Biography

Speaker
Wasfy Obeidat / Jordan University of Science and Technology
Jordan

Abstract

MgATP is supposed to be necessary for mKATP-channel activation by mKATP-channel openers (KCOs), but literary data regarding this are controversial. The aim of this work was to study the effect of diazoxide and pinacidil on mKATP-channel activity in brain mitochondria in the absence of MgATP. Without MgATP, mKATP-channel exhibited high sensitivity to KCOs, and full activation by ~1.5-2 times was shown at ≤500 nM of these drugs. To assure activation, mKATP-channel was specifically blocked by ATP in the presence of Mg2+. Neither Mg2+ nor ATP alone affected mKATP-channel affinity to KCOs, but MgATP shifted it to micromolar concentrations, which agreed with literary data. Conventional mKATP-channel activation by KCOs up to 100 µM in the presence of MgATP, and the block of the activated channel by glibenclamide and 5-hydroxydecanoate, gave the same estimate of maximal channel activity proving KCOs’ ability to elicit full activation of native channel at nanomolar concentration scale without MgATP. Based on the experiments, we reached the conclusion of high sensitivity of native mKATP-channel to KCOs and proposed a hypothesis that mKATP-channel might comprise some high affinity sites for KCOs binding in the absence of MgATP. The results of our study reveal novel not described earlier aspects of mKATP-channel interaction with KCOs that would help bring new insight into understanding mKATP-channel channel properties. As the molecular mechanism of mKATP-channel interaction with KCOs was not yet established, novel features of such interactions would help reveal unknown channel properties necessary for a design of more specific modulators of mKATP-channel activity.

Biography

Olga V Akopova has completed PhD in 1997 and a Dr. of Sciences degree in biochemistry in 2016. Now she is a principal investigator at Circulation department of AA Bogomoletz Institute of Physiology, NAS of Ukraine. Field of interests: mitochondrial potassium and calcium transport, KATP channel, impact of Ca2+ and K+ transport on mitochondrial bioenergetics and metabolism. Olga V Akopova is an author of a series of research works devoted to the study of bioenergetic and functional effects of mitoKATP channel opening in brain and liver mitochondria. Her works disclosed several novel aspects of physiological functions of mKATP channel showing the contribution of ATP-sensitive K+ transport in the modulation of transmembrane K+ exchange (K+ cycle), mitochondrial oxygen consumption and membrane potential, the modulation of Ca2+ uptake, ATP synthesis and ROS production. She is an author of about 25 research works cited in MEDLINE and Scopus.

Speaker
Olga V Akopova / AA Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine
Ukraine

Abstract

The objectives of this work were: implementation of a new pharmaceutical technique to improve aqueous solubility and thus dissolution, enhancement of drug permeation through adsorption onto nano-sized silica, andfinally formulation of a controlled release tablet loaded with glimepiride (GLMP).Different polymers were used to enhance aqueous GLMP solubilityof which a saturated polymeric drug solution was prepared and physically adsorbed onto silica. An experimental design was employed to optimize the formulation parameters affecting the preparation of GLMP matrix tablets loaded with physically modified drug. A compatibility study was conducted to study interactions among components. Scanning electron microscope (SEM) of the optimized tablets was performed before and after they were placed in the dissolution medium. An in vivo study in human volunteers was performed with the optimized drug-loaded matrix tablets,whichwere compared to pure and marketed drug tablets. The results revealed the successful improvement of aqueous drug solubility. All of the studied formulation factors significantly affectedCarr’s compressibility index, drug release after 1 h and the time required for 85% release.No significant interaction was detected among components. SEM confirmed the effect of the polymer and thus release of the drug via diffusion mechanism. The volunteer group administered the optimized GLMP tablet exhibited higher drug permeation, longer t½ and controlled drug release compared to other groups. It could be concluded that amatrix tablet loaded with a physically modified drug form could represent a key solution for drugs with inconsistent dissolution and absorption profiles.

Biography

Dr. Tarek travelled to USA in 2008 where he joined Texas A&M health science center (TX, USA) as a PhD visiting scholar. During that period Dr. Tarek got an excellent knowledge in the development of novel drug delivery (in situ gel and in situ microparticles), HPLC analysis for both in vitro and in vivo study. After completing his PhD study, he came back to Egypt in 2010 where he did make his defense within two months and joined the department of pharmaceutics and industrial pharmacy as lecturer in March 2010. Over his years of experience, Dr. Tarek gained an excellent teaching skill of both practical and academic theoretical pharmaceutical courses. He got academic teaching responsibilities at Al-Azhar university (2010-2011), the Egyptian Russian University (2010-2011) and currently during his work at King Abdulaziz University. His teaching experiences has been extended to the postgraduate study through teaching Nanotechnology, Novel drug delivery systems and Selective topics for master students. Beside the teaching skill, Dr. Tarek has a lot of international publications in the pharmaceutical scientific research journals such as: International Journal of pharmaceutics, European Journal of Pharmaceutical Sciences, Journal of Pharmaceutical Sciences, International Journal of Nanomedicine, Drug Design Development and Therapy Journal, Journal of Liposome Research, Journal of Microencapsulation, AAPS PharmSciTech, Drug Development and Industrial Pharmacy, Pharmaceutical Development and Technology, International journal of drug delivery and others.

Speaker
Tarek Abdelnapy Ahmed / King Abdulaziz University
Kingdom of Saudi Arabia

Abstract

Electrocardiogram and arterial pressure measurements were studied under acute exposures to WIFI (2.45 GHz) during one hour in adult male rabbits. Antennas of WIFI were placed at 25 cm at the right side near the heart. Acute exposure of rabbits to WIFI increased heart frequency (+22%) and arterial blood pressure (+14%). Moreover, analysis of ECG revealed that WIFI induced a combined increase of PR and QT intervals. By contrast, the same exposure failed to alter maximum amplitude and P waves. After intravenously injection of dopamine (0.50 ml/kg) and epinephrine (0.50 ml/kg) under acute exposure to RF we found that, WIFI alter catecholamines (dopamine, epinephrine) action on heart variability and blood pressure compared to control. These results suggest for the first time, as far as we know, that exposure to WIFI affect heart rhythm, blood pressure, and catecholamines efficacy on cardiovascular system; indicating that radiofrequency can act directly and/or indirectly on cardiovascular system.

Biography

Speaker
SAILI LINDA / Université Badji Mokhtar Annaba
Algeria

Abstract

Chronic Obstructive Pulmonary Disease (COPD) and bronchial asthma are obstructive lung diseases (OLDs) with high incidence and prevalence. COPD is a progressive inflammatory disorder mainly affecting the small airways. The disease is characterized by airway inflammation, increased airway resistance, fibrosis, mucus hyper-secretion, and parenchymal lesions with reduced elastic recoil and loss of alveolar attachments, leading to airflow limitation. It has been estimated that ∼40 million people in Europe are living with COPD and ∼60% are having significantly impaired lung function. Bronchial asthma is also a chronic inflammatory disease of the airways characterized by variable and recurring symptoms, local inflammation, reversible airflow obstruction, and bronchospasm. Asthma is a prevalent chronic disease (affecting ∼30 million people in Europe. It has a high burden of morbidity especially if not controlled. The asthma–COPD overlap syndrome (ACOS) has been defined as the coexistence of increased variability of airflow in a patient with incompletely reversible airway obstruction. According to the Global Initiative of Asthma (GINA), ACOS is not a single disease. Its risk factors are shared with asthma and COPD, including development in the later decades of life, airway hyper-responsiveness, often tobacco smoke exposure, and progressive loss of lung function. ACOS may also be characterized by more frequent and severe exacerbations as well as eosinophil and/or neutrophil cells in sputum as compared to asthma or COPD alone. There are several studies showing that vitamin D deficiency is common in COPD and associated with worse lung function parameters. In asthma, higher vitamin D comes together with fewer exacerbations, and the supplementation of the vitamin may be effective in the prevention of the exacerbations. High vitamin D levels were associated with improved lung function, better glucocorticoid response, and less airway hyperactivity in asthmatic patients. Both in COPD and asthma, vitamin D has the potential to influence the onset of the disease by impacting on the lung in utero and early in life. Vitamin D deficiency is a common feature of ACOS patients in a continental climate country and is similarly low as seen in severe COPD patients. 25(OH)D level showed association with lung function parameters and Asthma Control Test in old patients. ACOS patients have much worse quality of life measures when using COPD Assessment Test (CAT) and Asthma Control Test (ACT), and data on simultaneous quality of life measurements are important. Although vitamin D deficiency correlated with ACT and CAT scores in ACOS patients, this association was not present in asthma or COPD groups. Monitoring 25(OH)D level in ACOS and patient-related quality of life measurements are very important. Since Vitamin D deficiency is treatable, future studies examining the effect of supplementation are needed.

Biography

Aya r. lafta has completed her Msc program from Ajman University of Science & Technology, UAE. She is pharmacist in charge at Medicina pharmacy-UAE .She has published 4 papers in reputed journals.

Speaker
Aya Rahman Lafta / Medicina pharmacy
UAE

Abstract

Background and the purpose of study. At present, pharmaceutical researchers are focusing on instantaneous intraoral dispersible technologies as novel drug delivery systems because; they have outstanding advantages over the traditional oral and parenteral routes of drug administration. Some essential natural drugs have low oral bioavailability due to extensive first pass metabolism and pre systemic degradation in the gastrointestinal tract. Aim. Now, a cheap rice paper Intraoral Dispersible Film (IDF) has been developed Objectives. In this study, formulation was optimized using the experimental factorial design. The IDFs were loaded with model, natural, anti-cancer drugs, Resveratrol and Curcumin with low oral bioavailability. Methods. They were evaluated for thickness, folding endurance, swelling behaviour, among others. These related to their drug release properties. Permeation was evaluated using the pig mucosal membrane mounted on a Franz diffusion cell. Taste testing was done to determine acceptability using a taste panel. Results and Discussions. Sixteen formulations showed variations in their profiles. Formulation 16 proved optimal. The dissolution rate at steady state concentrations of Resveratrol was 29mg per second and the Permeability coefficient was 389 mg/sec.cm2. Curcumin dissolution rate at steady state concentrations was 0.25mg per second and the Permeability coefficient was 42.71 mg/sec.cm2. Resveratrol permeability rate was 0.42 mg/sec. and that of Curcumin was 0.14 mg/sec. Resveratrol Flux was 0.21 mg/sec./cm2. Curcumin Flux was 0.14 mg/sec. / cm2. Drug entrapment was 80% for both molecules. The 20 mg of Resveratrol and Curcumin dissolved in 47.6 sec. and 71.4 sec. respectively. In this study, after permeation, a concentration of 6.73mg/ml of Resveratrol and 0.061mg/ml of Curcumin were detected after 2 hours of the experiment on administering only 20 mg of each of the drugs suggesting that Curcumin is 100 times less permeable than Resveratrol. The release profile was a “Burst release”. On contrast, Curcumin oral dose of 2 g/kg to rats yielded 1.35±0.23 µg/ml in 0.83 hours and in humans, given the same dose yielded either undetectable or extremely low (0.006±0.005 µg/ml after 1 hour in blood. Two separate mono-glucuronide metabolites yielded a Cmax of ~7.5 µM following a single 5.0 g oral dosage of Resveratrol. Conclusion. The key finding was, ex vivo release profiles of the optimized formulation revealed first order release and later zero order. Therefore, it is evident that rice paper IDF could efficiently deliver natural drugs into the systemic circulation intraorally. However, further studies need to be performed to prove increased bioavailability in human subjects

Biography

Mr. Mukasa Eliphaz is a Ugandan National and has worked at Medipharm Industries EA Ltd. Uganda factory as a Production supervisor for 15 years. He is specialized in cGMP and Oral Rehydration Salts Manufacture. He has studied at Mulago National Referral Hospital School of Dispensing for a Higher Diploma Pharmacy in 1988. He taught at Mulago Hospital Paramedical School for 2 years. He has attended a Clinical Instructor’s course at Mbale Health Manpower Development Centre in 1999 and worked as an Assistant Drugs Inspector at Uganda National Drug Authority for 7 years. He has also attended NIPER Chandigarh India for Assessment of quality of Pharmaceuticals. He did his B Pharm in 2012 at Nelson Mandela Metropolitan University (NMMU) Port Elizabeth South Africa and his M Pharm at the University of The Witwatersrand Johannesburg South Africa in 2016. He has worked at Johannesburg General Hospital Charlotte Maxeke for his Pharmacist Internship in 2013. He is an Honorary member of the Golden Key International South Africa. He presented his Research work at the 11th. World Drug Summit in Baltimore Maryland USA, 2017. Presently, he is doing his Pharmacist community service in the rural areas at Nessie Knight Hospital Sulenkama Qumbu in the Eastern Cape South Africa, where he is also a member of the clinical audit team.

Speaker
Mukasa Eliphaz / Nessie Knight Hospital
Eastern Cape South Africa

Abstract

Rifaximin, an oral antibiotic marketed as tablets, does not have dissolution method described neither in official compendiums nor in literature. This lack of knowledge blinds the real rate of drug availability. Thus, all potentialities of the active principle are not enough if it is trapped in its formulation or it is released erroneously The absence of dissolution method can regress the action of the drug to an adjuvant. Therefore, the objective of this study was to develop and validate a successful dissolution method for evaluation of rifaximin tablets. The method contemplated the parameters for linearity, selectivity, precision, accuracy and robustness. It was considered appropriate for evaluation of tablets of rifaximin 200 mg, using paddle apparatus, 50 rpm and 900 mL of acetate buffer pH 5.0 + 0.2 % SLS as dissolution medium. The method presented is useful and applied for the routine quality control of tablets of rifaximin.

Biography

Ana Carolina Kogawa graduated in Pharmacy-Biochemistry in 2008, and received her Master’s in 2012 and PhD in Pharmaceutical Sciences in 2015 from the Universidade Estadual Paulista – UNESP, Brazil. She has experience in managing people, lectures, quality tools and pharmaceuticals activities of industry with emphasis on quality control. She has published more than 30 papers in renowned journals and has been serving as a reviewer of manuscripts in more than 10 international journals.

Speaker
Ana Carolina Kogawa / Univ Estadual Paulista
Brazil

Abstract

Dutasteride is a selective inhibitor of both types of 5α-reductase enzyme which converts testosterone to dihydrotestosterone. Therefore, the treatment of benign prostatic hyperplasia (BPH) is performed by inhibiting 5α-reductase. Dutasteride indications are BPH and men's alopecia, and marketed as a capsule dosage form. In many aspects tablets are better than capsules, for instance the size, shape, appearance, cost and it is suitable for large scale production. Moreover, tablets can be cut into two pieces with more patient compliance and chemical and microbial stability. The purpose of this study is to prepare dutatasteride 0.5 mg tablet by direct compression method. The suitable excipients were lactosespray dried, microcrystalline cellulose and magnesium stearate as lubricant. All formulations were evaluated trough, bulk density, hardness, weight variation, powder flow ability, friability, disintegration, dissolution and content uniformity test. Assay method and determination of dutasteride in tablet and raw material was developed using UV spectrophotometer at wavelength 240 nm. The linearity of calibration curve was established in concentration 5-50 μg/ml. After physicochemical evaluation of different formulations, the two optimised formula have been selected. Both formulations were indicated acceptable results in weight variation between 100-110 mg, dimension approximately 7.20 × 2.20, disintegration time ranged between of 4-8 min, dissolution test indicated 90% of drug content was released less than 45 min.

Biography

Speaker
Mozhdeh Hemati / Kermanshah University of Medical Sciences
Iran

Abstract

Methadone is a synthetic opioid medication best known for its use in the treatment of opioid dependence. Methadone is also an effective analgesic agent, potentially with increased efficacy in the setting of neuropathic pain. Preclinical research has studied and established that many nanoparticle (NP) characteristics, such as size, shape, surface properties and drug loading, can affect therapeutic efficacy, biodistribution, pharmacokinetics and toxicity of NP-drug formulations. This study were performed to analysis the effect of methadone nanoparticles (MNP) on the proliferation and cell viability of MCF-7 cells. MCF-7 cells were cultured in DMEM culture medium. This culture was divided into 2groups: (1) Culture that treated with methadone dose, (2) Culture that treated with MNP. There were six treatments including: control group (culture without any treatment, treatment i), cultures that treated with doses 20,40, 80, 160 and 320 µg (treatments ii-iv) of methadone and MNP respectively. The cells proliferation was assessed by MTT test and cell viability was assessed by LDH test. In group 1, methadone in treatments ii and iii was not toxic and increased the proliferation of cells compared with control treatment (p<0.05). Methadone in treatments iv to vi were toxic and decreased the cell viability and proliferation of cells compared with control treatment (p<0.05). In group 2, MNP in treatments ii to iv, were not toxic and increased the proliferation of cells compared with control treatment (p<0.05). MNP in treatments v and vi were toxic and decreased the cell viability and proliferation of cells compared with control treatment (p<0.05). In group 2, the cell viability and cell proliferation of MCF-7 cells in treatments ii to vi were increased compared with the same treatments of group 1, respectively (p<0.05). It can be concluded that the MNP at low concentration (20-80 µg) can increase MCF-7 cell proliferation, and was found nontoxic to cells.

Biography

Speaker
Maedeh Tahvilian / Kermanshah University of Medical Sciences
Iran

Abstract

The objective of this work isdeveloping more effective and promising mucoadhesive in situ gel nasal preparation for ondansetron HCl (OND-HCl) using nanoemulsion (NE) technology and study all the parameters that can optimize the final formula and compare it’s in vivo results to nasal in situ gel (IG) formula prepared using the conventional method. Screening of oils and emulsifiers was performed on the basis of OND-HCl solubility and NE area of emulsifier ratio (Smix), respectively. Pseudo-ternary phase diagrams were constructed by aqueous titration method. The optimized NE formula was prepared by ultrasonic emulsification and applied to prepare in situ nanoemulsion gel (ING) using poloxamer 407 as thermoreversible polymer, chitosan and HPMC E15 as mucoadhesive polymers. The six ING formulas were characterized for pH, osmolarity, gelation temperature, spreadability, mucoadhesive force, viscosity, in vitro drug release and in vivo study. The pH, osmolarity, gelation temperatures, spreadability,mucoadhesive force for the selected ING formula (F6) were 5.8, 106.06 mOsmol/l (0.62%), 33 ºC, 5 cm and 7372.21 dyne/cm² respectively. The in vitro drug release study of F6 showed 100% after 6 h. In-vivo studies showed significant increase in Cmax and AUC (P < 0.001) for the selected ING (F6) in comparison to conventional IG. This results indicated the possibility of application of NE technology in the formulation of ING that has good mucoadhesive force to prolong the residence time of the drug in the nose and improves drug absorption.

Biography

Speaker
Nidhal Khazaal Mareai Aalhasan / Mustansiriyah university
Iraq

Abstract

Background and the purpose of study. At present, pharmaceutical researchers are focusing on instantaneous intraoral dispersible technologies as novel drug delivery systems because; they have outstanding advantages over the traditional oral and parenteral routes of drug administration. Some essential natural drugs have low oral bioavailability due to extensive first pass metabolism and pre systemic degradation in the gastrointestinal tract. Aim. Now, a cheap rice paper Intraoral Dispersible Film (IDF) has been developed Objectives. In this study, formulation was optimized using the experimental factorial design. The IDFs were loaded with model, natural, anti-cancer drugs, Resveratrol and Curcumin with low oral bioavailability. Methods. They were evaluated for thickness, folding endurance, swelling behaviour, among others. These related to their drug release properties. Permeation was evaluated using the pig mucosal membrane mounted on a Franz diffusion cell. Taste testing was done to determine acceptability using a taste panel. Results and Discussions. Sixteen formulations showed variations in their profiles. Formulation 16 proved optimal. The dissolution rate at steady state concentrations of Resveratrol was 29mg per second and the Permeability coefficient was 389 mg/sec.cm2. Curcumin dissolution rate at steady state concentrations was 0.25mg per second and the Permeability coefficient was 42.71 mg/sec.cm2. Resveratrol permeability rate was 0.42 mg/sec. and that of Curcumin was 0.14 mg/sec. Resveratrol Flux was 0.21 mg/sec./cm2. Curcumin Flux was 0.14 mg/sec. / cm2. Drug entrapment was 80% for both molecules. The 20 mg of Resveratrol and Curcumin dissolved in 47.6 sec. and 71.4 sec. respectively. In this study, after permeation, a concentration of 6.73mg/ml of Resveratrol and 0.061mg/ml of Curcumin were detected after 2 hours of the experiment on administering only 20 mg of each of the drugs suggesting that Curcumin is 100 times less permeable than Resveratrol. The release profile was a “Burst release”. On contrast, Curcumin oral dose of 2 g/kg to rats yielded 1.35±0.23 µg/ml in 0.83 hours and in humans, given the same dose yielded either undetectable or extremely low (0.006±0.005 µg/ml after 1 hour in blood. Two separate mono-glucuronide metabolites yielded a Cmax of ~7.5 µM following a single 5.0 g oral dosage of Resveratrol. Conclusion. The key finding was, ex vivo release profiles of the optimized formulation revealed first order release and later zero order. Therefore, it is evident that rice paper IDF could efficiently deliver natural drugs into the systemic circulation intraorally. However, further studies need to be performed to prove increased bioavailability in human subjects

Biography

Mr. Mukasa Eliphaz is a Ugandan National and has worked at Medipharm Industries EA Ltd. Uganda factory as a Production supervisor for 15 years. He is specialized in cGMP and Oral Rehydration Salts Manufacture. He has studied at Mulago National Referral Hospital School of Dispensing for a Higher Diploma Pharmacy in 1988. He taught at Mulago Hospital Paramedical School for 2 years. He has attended a Clinical Instructor’s course at Mbale Health Manpower Development Centre in 1999 and worked as an Assistant Drugs Inspector at Uganda National Drug Authority for 7 years. He has also attended NIPER Chandigarh India for Assessment of quality of Pharmaceuticals. He did his B Pharm in 2012 at Nelson Mandela Metropolitan University (NMMU) Port Elizabeth South Africa and his M Pharm at the University of The Witwatersrand Johannesburg South Africa in 2016. He has worked at Johannesburg General Hospital Charlotte Maxeke for his Pharmacist Internship in 2013. He is an Honorary member of the Golden Key International South Africa. He presented his Research work at the 11th. World Drug Summit in Baltimore Maryland USA, 2017. Presently, he is doing his Pharmacist community service in the rural areas at Nessie Knight Hospital Sulenkama Qumbu in the Eastern Cape South Africa, where he is also a member of the clinical audit team.

Speaker
Mukasa Eliphaz / Nessie Knight Hospital
Eastern Cape South Africa

Abstract

Mulberry leaves has traditionally been cultivated for feeding larvae of Bombyx mori. It also have been known to be rich in 1-deoxynojirimycin (DNJ) which inhibits postprandial hyperglycemia by inhibiting -glucosidase in the small intestine, has anti-virus and anti-tumor activities. DNJ content is varied from mulberry variety, and leaf position and seasons. The variety change ranged from 0.03% to 0.39%, and leaf position followings shoots > young leaf > mature leaf. DNJ production from mulberry leaves could be significant improved by specific microorganism fermentation. Our study shown that Ganoderma lucidum to be the most effective microorganism to improvement of DNJ production by 2.7 folds. 40 mg/mL of DNJ extracts had an inhibition efficiency of 92.27% to Hela tumur cells, being able to eliminate tumor cell proliferation completely. And, the inhibitory effect of DNJ extracts on -glucosidase activity is similar to those of acarbose without significantly difference. Orally administration of DNJ extracts (1.5 g/kg) to mice, the increase of blood glucose levels after alloxan-induced was significantly suppressed. The mechanism of -glucesidase inhibition of DNJ extracts was competitive.

Biography

Zhongzheng Gui, professor at Jiangsu University of Science and Technology (JUST), China, is the chief of Laboratory of Hi-Tech Processing for Sericultural Resources. His experience in the area of Functional Natural Products, working mainly in: silkworm, mulberry and their biomedical applications. His research program covers various subject areas, including microbiology and biotransformation, and biomedical. He has published more than 30 papers in reputed journals and has been serving as an editorial board member of repute.

Speaker
Zhongzheng Gui / Jiangsu University of Science and Technology
China

Abstract

The aim of this study was to prepare lipid based solid dispersion using hot melt extrusion technology with TPGS® to enhance solubility of various poorly drugs and its in vitro outcomes were interpreted by in silico materials to predict in vivo behavior. The drug (40% w/w), Kollidone VA® 64(20%), TPGS® (32%) and Cremophor® (8%) were simply blended by mixer. The following blends were extruded using a twin screw extruder (11mm-Hygenic, ThermoFisher Scientific) at screw speed of 100 rpm and temperature range of 100~170℃. Simplus’ PBPK simulation program, GastroplusTM was used and basic provided drug database used for physiological data. Celecoxib HME solid-dispersion was showed 4 times higher drug release than pure celecoxib and hot melt extruded fenofibrate was also solubilized, while pure cilostazol showed higher drug release behavior than extrudate in 0.5% of SLS solution. All three drugs have known as low solubility in water and high log P values, but drug release behaviors were different in dissolution media containing 0.5% SLS. Hot melt extruded based solid dispersion had 10 times larger particle size, 2 times smaller particle density and higher solubility in diluted water. Even though hot melt extruded solid dispersion had higher solubility in diluted water, simulation results showed lower drug release pattern of Cilostazol because it is engaged with particle size rather than water solubility. Thus, solid dispersion of cilostazol has decreased 26.2% of bioavailability, 27.7% of Cmax and 28.3% of AUC comparing with API powder. This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIP) (No. 2017R1C1B1003590).

Biography

Jun-Bom Park has completed his PhD from College of Pharmacy, Kangwon National University, Republic of Korea and postdoctoral studies from Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, USA. He has published more than 50 papers in reputed journals.

Speaker
Jun-Bom Park / Sahmyook University
Korea (South)

Change Color