AIDS was first recognized by the United States Centers for Disease Control and Prevention (CDC) in 1981.Between its discovery and 2014 AIDS has caused an estimated 39 million deaths worldwide. It is known that HIV-1 infects CD4+ T cells, macrophages, and dendritic cells through the binding with CD4 and/or DC-SIGN molecules. So we expressed those genes on the surface of red blood cells which are destroyed regularly in the spleen after their lif-span. To do this, we replaced the CMV promoter of pLenti6.3/DEST vector with beta globin promoter to express human gene in RBC and obtained pLenti6.3/beta globin expression vector. In addition, the CD4 gene (1.3 kb) which obtained from human leukocytes and cloned into pCR8 cloning vector, named pCR8_CD4 vector. TheLR recombination reaction had been done between pLenti6.3/beta globin expression vector and pCR8_CD4 vector to insert CD4 gene, named pLenti6.3_beta globin_CD4 expression vector. CD4 gene was expressed in erythroblast which is stem cell of RBC by transfecting the pLenti6.3_beta globin_CD4 expression vector. The same procedure had been done to construct the DC-SIGN expressed vector, named pLenti6.3_beta globin_DC-SIGN expression vector. Those two molecules were expressed well on the surface of erythroblasts in FACS analysis. In near future, we’d like to challenge expressing CD4 and DC-SIGN gene in erythroblast of humanized SCID mice to evaluate cleaning up effect of HIV-1
Hyeong Woo Lee is serving as R&D CEO of Scorpiogen Co. which is developing diagnostic tools for infectious diseases since 2017. During 2006-2009, he was a Postdoctoral Associate at Massachusetts General Hospital, Harvard Medical School. He received a B.S from Kyung Hee University in 1989, and an M.S. and Ph.D. in microbiology and molecular biology from the same university in 1994 and 1999, respectively. From 2013 to 2014 he worked at University of Florida as Senior Biological Scientist.
African swine fever (ASF), a devastating disease of swine, was first reported in Africa and has since spread to Asian, European, and South American countries. The disease is caused by African swine fever virus (ASFV), the only member of the family Asfarviridae and the genus Asfivirus, and the only known DNA arbovirus. The epidemiology of ASF is complex. It can be transmitted by direct contact or by contact with infectious secretions/excretions and ASFV can colonize both pig pens in domestic areas and burrowing wild mammals. There was no ASF case reported in South Korea, but it is urgent need developing rapid and simple diagnostic methods to prevent the imported from other outbreak countries.In this present study, we have developed rapid molecular diagnosis methods to diagnose ASFV using Reverse Transcription-Recombinase Polymerase Amplification (RT-RPA) method. Primers and the exo probe targeting the conserved region of the P72 gene of ASFV were designed and the reaction was run on the T8 UV scanner device.For ASVF specific forward and reward primers were consisted of 35 nucleotides each and probe consisted of 51 nucleotides which included FAM-dT, THF, and BHQ-dT. The expected gene amplification size was 141 bp.TheASFV specific primer and probe set detected only ASFVinfected samples.We showed that the amplified product could be detected in less than 20 min and that the detection limit was 0.0001pg DNA/reaction. Over 0.01 pg DNA of ASFV samples were detected within 10 min. Therefore, the RPA assay, which is simple, cost-effective, and fast, is a promising alternative to real-time PCR for ASFV detection in South Korea.
Yoon Hee Bae serve as R&D assistant in Konkuk University since 2017. During 1990-1995, she was a nurse at Hyundai AsanGeneral Hospital. She received a B.S from Chung AngUniversity in 1990.
In Colombia, floriculture represents an important economic activity in which the main activity is focused over the chrysanthemum, roses and carnation production and exportation, but other no-traditional species such as Heliconia are considered. This cultural activity does not exceed 30 years, as a result, several technical requirements have not been addressed so far, e.g. differential response to environmental conditions and its consequence over productivity. Stability and adaptability studies described in this manuscript help in the identification of varieties with predictable behavior and its response to climatic variations. These kind of studies allowed the evaluation of 10 promissory commercial cultivars in three different locations throughout 27months, employing the effect of main additive and multiplicative interaction model (AMMI). AMMI method is a multivariate method that represents in an effective manner the components of the interaction by genotype by environment. This evaluation helped to identify commercial cultivars better adapted to every climate condition. From evaluated material, the best adapted and more stable varieties in every environment were H. bihai cv Lobster salmon, H. orthotricha cv Arco iris, H. orthotricha cv Tricolor and H. bihai cv Peach Pink. The last mentioned specie, presented low flower production despite its excellent sprouting.
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