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Plenary Talks

Abstract

We will present our long-term team(s) experience during forty years in bioscience and biotechnology. Lecture includes some of our selected innovations as follows: (1) Discovery of natural photosensitizers and their potential use from bench to clinical applications in diagnosis and treatments of cancer using photodynamic therapy (PDT) by Lasers. (2) Biochemical role of intra-vaginal sildenafil as novel antiabortive agent, also its application as novel treatment with Silymarin for treatment of PCOS patients undergoing IVF for first time. (3) Novel applications of natural phytochemicals and their new Nanoformulations in treatment of cancer and diabetes. (4) Novel biomarkers for cancer diagnosis. (5) Novel management of diabetes by means of stem cell biotechnology

Biography

Professor Mohamed El-Far, worked in biochemistry field for 40 years, published over 90 peer-reviewed papers. He received Fulbright and British council fellowships several times as well as German DAAD grant to establish PDT Program at Munich, also received US-AID grant to establish PDT unit in Egypt. He is serving on the editorial boards and Hon. Editor to four international journals. He acts as UNESCO expert in science and technology. Dr. El-Far served as visiting professor to University of California as well as Utah laser center also Mayo clinic for several years. He also served as a visiting professor to Cardiff and Swansea Universities, UK. He is a member of International Photodynamic Association and Royal Society of Chemistry, UK. Selected and served as expert and consultant for biochemistry in the national committee of suprime council of Universities in Egypt, which is the highest nation honor. Recently selected as a member of higher Education and Research committee for Mansoura University. Selected by the International Biographical Centre, Cambridge, England to be among top 100 scientists 2012. Received honary doctorate of Letters from International Biographical Centre, Cambridge, England. Selected on the list of global speakers as chairman, a keynote speaker and honorable guest for several international conferences in cancer and diabetes as well as other recent biomedical applications as stem cells and nanotechnology. Selected as Mansoura university person of the year 2017 / 2018 and awarded certificate of recognition and Mansoura University Shield.

Speaker
Mohamed El-Far. / Mansoura University, Egypt

Abstract

New paradigms in the research and development of nanocarbon thin films are providing the bases for new physics, materials science, chemistry, and biological processes, impacting a new generation of multifunctional biomedical devices. This talk will focus on discussing the science and technology of the new paradigm material named ultrananocrystalline diamond (UNCDTM) in thin film form and integration into a new generation of medical devices and implants as described below: UNCDfilms co-developed and patented by O. Auciello and colleagues are synthesized by novel microwave plasma chemical vapor deposition and hot filament chemical vapor deposition techniques using an Ar-rich/CH4 chemistry that produces diamond films with the smallest gran size demonstrated today (2-5 nm). The fundamental science underlying the synthesis and properties of the UNCD films and applications to devices will be discussed. The UNCD films exhibit the lowest friction coefficient (0.02-0.04) compared with metals (≥ 0.5) currently used in many prostheses (e.g., hips, knees), electrically conductive UNCD coatings with nitrogen in grain boundaries can enable a new generation of neural electrodes, UNCD coatings are extremely biocompatible, and have been demonstrated to provide superior scaffolds for embryonic cell growth and differentiation. Original Biomedical Implants (OBI-USA) and OBI-México, founded by Auciello and colleagues, are developing new generations of implantable medical devices based on the biocompatible UNCD coatings, namely: a) new generation of Li-ion batteries with ≥ 10x longer life and safer, using UNCD-based electrodes, membranes and inner wall battery case, enable next generation of defibrillator/pacemakers; b) new generation of implantable prostheses (e.g., dental implants, hips, knees, and more) coated with UNCD eliminates failure of current metal-based implants due to synergistic mechanical wear / chemical corrosion by body fluids; c) UNCD-coated polymer with brain neurons tailored stiffness enables next generation less invasive electrodes for neural stimulation; d) UNCD-based MEMS energy generation cantilever powered by biting heart cells to power new generation defibrillator/pacemaker; e) UNCD-based MEMS cantilevers have been demonstrated as high sensitivity biosensors.A key medical device where UNCD coating has made an impact is a UNCD-coated silicon based microchip implantable inside the eye as a key component of the artificial retina to return partial vision to people blinded by genetically-induced degeneration of photoreceptors (a device named Argus II is currently in the market by Second Sight, returning partial vision to people blinded by retinitis picmentosa).

Biography

Auciello graduated with honors with M.S. (1973) and Ph.D (1976) degrees in Physics from the Physics Institute “Dr. Balseiro” (Universidad Nacional de Cuyo-Argentina). EE-University of Córdoba-Argentina (1970). Postdoctoral-McMaster University, Hamilton, Canada (1977-1979); Researcher-University of Toronto-Canada (1979-1984), Associate Professor-NCSU-USA (1984-1988), Distinguished Scientist-MCNC-USA (1988-1996), Distinguished Argonne Fellow (1996-2012)-Argonne National Laboratory-USA. Currently, Auciello is Distinguished Endowed Chair Professor-University of Texas-Dallas, Materials Science and Engineering and Bioengineering. Auciello is directing basic and applied research programs on multifunctional oxide [ferroelectric (piezoelectric) and high-K dielectrics thin films], and nanocarbon thin films (novel ultrananocrystalline diamond (UNCDTM) and graphene thin films and application to industrial, high-tech, and medical devices. The UNCD film technology is commercialized for industrial products by Advanced Diamond Technologies, founded by Auciello and colleagues, (2003, profitable in 2014), and by Original Biomedical Implants (OBI-USA, 2013) and OBI-México (2016), founded by Auciello and colleagues, for a new generation of superior medical devices and implants. Auciello has edited 20 books and published about 500 articles in several fields, holds 20 patents, He is associate editor of APL and Integrated Ferroelectrics, He was President of the Materials Research Society (2013) Auciello is Fellow of AAAS andMRS, and has numerous Awards. and He has a H-Index of 68

Speaker
Orlando Auciello / University of Texas-Dallas, USA

Abstract

Ploem’s invention of multi-wavelengths epi-illumination fluorescence microscopy (1967) and the invention of reflection-contrast microscopy (1975), would not have been possible without the advice and support of researchers in biology and scientists from the optical industry. A typical example is the scientific input from W. Hijmans and H. R. Schuit (1981), who are among of the first researchers who tried to detect two different fluorescence markers (FITC and TRITC), in the same cell. They had a fluorescence microscope available with dark field illumination with UV (365 nm) light . It was impossible for them to demonstrate these markers individually. The advice they gave Ploem, triggered an intensive cooperation of Ploem with Schott AG in Germany, a firm that had an expert knowledge about interference filters. Schott did develop quite a series of novel dichromatic (dichroic) mirrors. All these dichromatic mirrors had to be tested extensively on biological specimens in Ploem’s laboratory. This resulted in the interesting new fact that FITC could now for the first time be excited with blue light (490 nm) resulting in green fluorescence observed at 520 nm and very little disturbing autofluorescence. The possibility to excite fluorescence with green light (530 nm), was a novel technique, not reported before in fluorescence microscopy. This resulted in the emission of red (560 nm) fluorescence of TRITC. To enable a large variety of biological fluorescence applications, many new dichromatic mirrors had to be developed. This resulted in more than 40 different types of fluorescence filter cubes. Four or eight of these can be easily inserted in a fluorescence epi-illuminator. This technology development was so significant that quite a number of USA firms started to produce fluorescence filter cubes (Chroma, Semrock, Omega optical, Thor Labs, Delta optical and Alluxa). The possibility to detect now several fluorescent markers in a biological specimen led to the surprising result that with 8 selected filter cubes, inserted in a fluorescence epi-illuminator, all the individual human chromosomes could be finally identified with fluorescent color coding technology, in stead of performing the difficult visual identification! Brumberg (1959), who developed the first epi-fluorescence microscope with a dichromatic mirror for ultraviolet excitation (365 nm), was probably not informed by biologists of the urgent need for avoiding autofluorescence of tissues by UV excitation, and over the possibility to use a much more effective excitation of the widely used fluorescence markers FITC and TRITC (developed by A. Coons, 1941). His invention was not marketed by major microscope firms like LEITZ/LEICA and ZEISS. Several other examples of the need for an inventor to contact academia and industry specialists, that would stimulate biological technology developments, will be reported in this lecture.

Biography

J. S. Ploem, Ploem received and M.D. from the university of Utrecht, and a Ph. D. from the university of Amsterdam. At Harvard university he obtained a M.P.H degree. He was a professor at Leiden university, and a visiting professor in Brussels and Beijing. An extensive biography is published as achapter in Wikipedia (title: Johan Sebastian Ploem).

Speaker
Johan Sebastian Ploem / Purdue University Cytometry Laboratories, Netherlands

Keynote Talks

Abstract

According to data of 13C, 1H NMR, 2D heteronuclear 1H/13C HSQC, 1D NOE and 2D DOSY experiments the main chemical constit¬uent of high molecular (>1000 kDa) water-soluble preparations from different species of two genera Symphytum and Anchusa (Boraginaceae family) was found to be poly[oxy-1-carboxy-2-(3,4-dihydroxyphenyl)ethylene] or poly[3-(3,4-dihydroxyphenyl)glyceric acid] (PDPGA). The polyoxyethylene chain is the backbone of this polymer molecule and 3,4-dihydroxyphenyl and carboxyl groups are regular substituents at two carbon atoms in the chain. The repeating unit of this regular polymer is 3-(3,4-dihydroxyphenyl)glyceric acid residue. Then the monomer and methylated derivative of PDPGA were synthesized and compared their pharmacological properties. The racemic monomer was synthesized for the first time via Sharpless asymmetric dihydroxylation of trans-caffeic acid derivative using an potassium osmiate catalyst. Methylated derivative of PDPGA was synthesized via ring opening polymerization of 2-methoxycarbonyl-3-(3,4-dimethoxyphenyl)oxirane using a cationic initiator. PDPGA is endowed with intriguing pharmacological activities as anticomplementary, antioxidant, anti-inflammatory, burn and wound healing and anticancer properties. PDPGA and its synthetic monomer exerted anticancer activity in vitro and in vivo against androgen-dependent and androgen-independent human prostate cancer (PCA) cells via targeting androgen receptor, cell cycle arrest and apoptosis without any toxicity, together with a strong decrease in prostate specific antigen level in plasma. However anticancer efficacy of PDPGA against human PCA cells is more compared to its synthetic monomer. Methylated synthetic analogue of PDPGA did not show any activity against PCA. Overall, this study identifies PDPGA as a potent agent against PCA without any toxicity, and supports its clinical application.

Biography

Vakhtang Barbakadze has his expertise in isolation and structure elucidation of a new series of plant polyethers. He has completed his Ph.D and D.Sci. in 1978 and 1999 from Institute of Organic Chemistry, Moscow, Russia and Institute of Biochemistry and Biotechnology, Tbilisi, Georgia, respectively. He is the head of Department of plant biopolymers and chemical modification of natural compounds at the Tbilisi State Medical University Institute of Pharmacochemistry. 1996 and 2002 he has been a visiting scientist at Utrecht University, The Netherlands, by University Scholarship and The Netherlands organization for scientific research (NWO) Scholarship Scientific Program, respectively.

Speaker
Dr. Vakhtang Barbakadze / Tbilisi State Medical University I.Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia

Abstract

The biological effect of photodynamic therapy(PDT)is based on the ability of cells to generate the reactive oxygen species(ROS) by the influence of low-intensity radiation with wavelengths of the visible spectrum. The clinical application of PDT is limited due to the lack of effective photosensitizers. Porphyrins and metalloporphyrins are currently widely used as photosensitizers, but poor solubility, rapid blood clearance and weak internalization reduce their antitumor efficacy. While the chlorophyll and other tetrapyrrolic compounds which may are structurally related to heme and bilirubin are among molecules which are responsible for the redox environment of tumor cells.The prooxidative and antiproliferative effects of liposomal form chlorophyll – chlorophyllin and drug "Chlorophyll OXY", it’s including as active component, were studied in the models “in vitro” and “in vivo”.Chlorophyllinprovides good absorption, digestibility, stability and accurate transport to target cells. Ithas been shown to produce antiproliferative effects inEhrlich's ascites carcinoma (EAC) cell linein a dose-dependent manner. Chlorophyllin have been observed to increase production ROS and enzymatic activity of EAC cells, including the production of mitochondrial/whole-cell ROS, and alter the ratio of reduced-to-oxidized glutathione. Importantly, "Chlorophyll OXY"-mediated suppression of EAC cell viability has been replicated in “in vivo” experiments, where its administration a resulted in the reduction of tumor size in mice. In conclusion, this data suggests that chlorophyllin mediated changes on the redox status of cancer cells leads to a decrease in its proliferation.

Biography

Natalia G. Plekhova is a Head of Central Researcher Laboratory Pacific State Medical University, Russia. She earned her PhD in Biology and has published over 120 peerreviewed journal articles. The theoretical positions as a new scientific achievement in the field of viral cytopathology, which is essential in the problem of research on the cellular and molecular interaction of cells and viruses, were developed. The role of innate immunity cells (macrophages and neutrophils, dendritic cells) in infections caused by RNA-containing viruses, the types of their cytopathogenic action and the form of viral infection of these cells were installed. Career interests: cell technologies in medicine, bioinformatics technologies, biocompatibility testing.

Speaker
Natalia G. Plekhova / Pacific State Medical University, Russian Federation

Abstract

will updated soon

Biography

Dr. Shailendra Kumar Tiwari completed his PhD at the age of 30 years from Rani Durgawati University, Jabalpur (M.P.), India. Presently he is working as a Senior Scientist and Head, Forest Genetics, Plant Propagation and Biotechnology Division of State Forest Research Institute, Jabalpur (Madhya Pradesh), India. He has published more than 70 research papers in National and International journal of repute. He has also published several technical bulletin related to forestry field. He has attended several international conferences particularly in the field of plant biotechnology. Dr. Tiwari has more than 33 year of research experience in the field of Tree Improvement, Forest Genetics, Plant Propagation and Plant Biotechnology.

Speaker
Shailendra Kumar Tiwari / Forest Research Institute, India

Abstract

Anandamide, a fatty acid neurotransmitter, controls "joy, bliss, and delight", and regulates neural generation of pleasure and motivation. It plays an important role in pain, appetite, and fertility. Anandamide is synthesized by N-arachidonoyl phosphatidylethanolamine and degraded primarily by the fatty acid amide hydrolase (FAAH) enzyme converting anandamide into ethanolamine and arachidonic acid. Since the impaired level of anandamide is associated with binge-eating disorder, stress-induced anxiety and anorexia nervosa, the detection of impaired levels of anandamide are important in biological fluids. Therefore, development of an electrochemical based devices to measure levels of anandamide is important in apparently healthy and persons suffering from effects of neurotransmitter imbalances. The use of nanomaterials provides efficient modification for electrochemical sensing of neurotransmitters. In the present work, the synthesized Imprinted Polymer of the molecule is coated on the nickel nanoparticle – MWCNT modified electrode effectively facilitating ultrasensitive detection of anandamide. An electrochemical sensing device decorated with nanomaterials is constructed through monomers Methacrylic Acid (MAA) and Methacrylamide (MetAM), cross linking agent Ethylene glycol dimethylacrylate (EGDMA) and polymerized through initiated Azobisisobutyronitrile (AIBN). The imprinted anandamide is removed and electrodeposited onto surface of a electrode. Once characterized by combination of varirous techniques including Electrochemical Study, FTIR, DPV, TGA and FE-SEM, the final sensor is developed. The sensor is further evaluated for its performance by limit of detection (LOD), linear range, percent recovery and precision in Blood plasma and Cerebrospinal Fluid (CSF) samples.

Biography

:- Dr. Nidhi Chauhan holds an Assistant Professorship at Amity Institute of Nanotechnology, Amity University, India. A PhD gold medalist with notable achievements in the area of Nanotechnology and Biosensors, Dr. Chauhan published more than 75 high quality research papers in International journals including including ACS Applied Materials and interfaces, Biosensor and Bioelectronics, RSC advances, Analyst, Analytica Chimica Acta and so forth. (Average Impact factor: 2.6, H-index:19 and total citation:1138). Dr. Chauhan persuaded to achieve many outstanding contributions in advance research by incorporating tools of nanotechnology for detection of various molecules and filed 13 Indian patents and 1 US patent. Her research work was highlighted by Nature India and Atlas of Scince. Indian Council of Medical Research, Government of India awarded Dr. Chauhan a prestigious Shakuntala Devi Amir Chand award for her scientific contribution in medical research. Further to her achievements, she is an Indo-Australia Career Boosting Gold Fellow by Department of Biotechnology to work with Nobel Laureate Prof. Barry J. Marshall at UWA, Australia. Dr. Chauhan was funded for her research as a Young Scientist by Department of Science and Technology and a Visiting Scientist at Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), India.

Speaker
Nidhi, Chauhan / Amity Institute of Nanotechnology, Amity University, Ind

Sessions:

Enzyme and Protein Engineering

Abstract

Nanoparticle-mediated gene therapy has been involved for therapeutic improvement in cancer. Recently mesoporous silica nanoparticles (MSNs) has drawn significant attention for potential biomedical applications, due to its attractive multifaced features such as huge surface area, tunable pore size, high drug loading capacity and biocompatibility. Globally lung cancer remained as the leading causes of death In the present study MSNs were synthesized and well characterized. MSNs were loaded with combined anticancer drug and functionalized with survivin siRNA.Our results revealed combinational approach is quite effective to achieve synergistic effect. This approach will shed new light on future design and applications for lung cancer therapy

Biography

Dr Fahima Dilnawaz completed her PhD from Utkal University, Bhubaneswar, India. Dr Dilnawaz’s commitment to research won her ITC fellowship from Hungarian Academy of Sciences, Postdoctoral fellowship from Dept of Biotech, Institute of Life Sciences, Bhubaneswar, International travel support from DST and grant awards in the form of Women Scientist from Dept. Sci. Tech. She is currently working as Women Scientist at Institute of Life Sciences, Bhubaneswar. To her credit she has published more than 30 scientific papers and review articles in reputed journals, 9 book chapters and 1 book. She has two patents to her credit. She has been serving as reviewer for various nanomedicinal journals as well as associated editorial board member of reputed journals.

Speaker
Fahima Dilnawaz / Institute of Life Sciences, India

Sessions:

biophysical cell communication

Abstract

This talk is about testing for an intercellular communication system that uses electromagnetic waves as a transmitter. Hence, it is about the original cell-phones. Indirect evidence has accumulated over the years that cells can communicate electromagnetically with each other. Experiments with the Ciliate Paramecium caudatum, a freshwater unicellular organism of the Eurasian plate, do support these findings as well. With a derivative of Gurwitsch’s early design (1923) for studying non-chemical cell communication, populations of Paramecia were chemically separated from each other by placing them into two cuvettes of different sizes, one standing in the other. Such pairs were exposed to each other during 48 hrs while they were in a black box. The effects of neighbouring populations as compared to their absence regard increased or decreased cell growth, energy uptake, density regulation and altered growth between (other) aquatic species. Evidence for one population compensating mortality in the other was found, too. Cells apparently use more than chemical signals for communication. They are, sensitive to external em-fields, generate these fields themselves, and inherit them, too. Hence, the results from investigating cellular em-fields are supposed to affect theories of life, diagnosis and therapy, ecology, environmental protection, and presumably evolutionary theory as well.

Biography

Dr. Daniel Fels received his Ph.D. degree regarding host-parasite interactions with Professor Dieter Ebert at the Zoological Institute of the University of Basel, Switzerland (2003) and had postdoctoral training in the laboratory of Professor Jacob Koella and Dr. Oliver Kaltz (CNRS, Université Pierre et Marie Curie, Paris, France). His primary field was evolutionary biology with research emphasis on host parasite interaction and transmission ecology. His works were published in the Archives of Hydrobiology, Behaviour, Oikos, Proceedings of the Royal Society and Parasitology. In 2006, he entered the developing field of physical signalling among cells. His early independent research (performed at former Swiss Tropical Institute and the CNRS) on electromagnetic signalling between cells was published in the April 2009 issue of PLoS ONE and in 2012 in Axiomathes. In 2009, he continued his independent research as Guest Scientist at the Institute of Botany, University of Basel. From this period followed the international conference Fields of the Cell (in 2012), the edition of the eBook Fields of the Cell (eds. Fels D, Cifra M, Scholkmann F) as well as his study on non-chemical cell communication across the species border (J Biophysics 2016) followed by a study on electromagnetic population density regulation (Scientific Reports, 2017) and the essay “The double-aspect of Life” (Biology, 2018). Fels is currently developing his private laboratory of biophysical cell communication (LBC). and Parasitology. In 2006, he entered the developing field of physical signalling among cells. His early independent research (performed at former Swiss Tropical Institute and the CNRS) on electromagnetic signalling between cells was published in the April 2009 issue of PLoS ONE and in 2012 in Axiomathes. In 2009, he continued his independent research as Guest Scientist at the Institute of Botany, University of Basel. From this period followed the international conference Fields of the Cell (in 2012), the edition of the eBook Fields of the Cell (eds. Fels D, Cifra M, Scholkmann F) as well as his study on non-chemical cell communication across the species border (J Biophysics 2016) followed by a study on electromagnetic population density regulation (Scientific Reports, 2017) and the essay “The double-aspect of Life” (Biology, 2018). Fels is currently developing his private laboratory of biophysical cell communication (LBC).

Speaker
Daniel Fels / Laboratory of biophysical cell communication, Switzerland

Sessions:

Bioscience

Abstract

Coexistence of distinct definitions of metabolic syndrome (MS) unifying the factors responsible for cardiovascular diseases and diabetes, impedes the analysis of interactions between their component features. In the general population the increasing incidence of subjects with insulin resistance and it sequels is observed. These individuals are characterized by normal weight and body mass index, that aggravate their identification and diagnostic. In parallel to main disorders associated with MS, many other abnormalities exist, i.e. disturbance of oxidant/antioxidant balance. The aim of the research was an evaluation of oxidative-antioxidative balance in blood of metabolically obese subjects with normal body weight, with an early and fully symptomatic form of metabolic syndrome. The oxidative/antioxidative balance was assessed based on activity of superoxide dismutase, catalase, glutathione peroxidase, paraoxonase activity and malondialdehyde (MDA) concentration in peripheral blood collected from all subjects. The oxygen metabolism disturbance, both as reactive oxygen form overproduction and abnormalities in effective antioxidative defence in persons with metabolic diseases was confirmed. It was that the oxidation/antioxidation disturbances are linked in ‘cause-result’ relation with cellular membranes labilization. In studied population the function of redox systems’ disruption was seen in advanced stage of diseases. Based on anthropometric and biochemical parameters, numerous abnormalities were shown. These abnormalities are not included in metabolic syndrome diagnostic criteria. This knowledge may be useful in the implementation of metabolic disorders prevention, particularly in metabolically obese persons with normal body mass.

Biography

Dorota M. Olszewska-Słonina, PhD, received her habilitation and actually work as associate professor at the Nicolaus Copernicus University, Poland. She obtained a diploma of post-graduate studies at Joseph Fourier, Grenoble, France. She is the head of Department of Pathobiochemistry and Clinical Chemistry (Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland). She has published 150 papers, including in reputed journals and has been serving as reviewer of few of them.

Speaker
Dorota Olzewska-slonina / Nicolaus corpernicus University, Poland

Abstract

A glycidyl epoxide, adduct of tung oil (ETO) was synthesized using glycidyl methacrylate (GMA) and tung oil and evaluated as potential bio-renewable replacements for bisphenol A based epoxy coatings. ETO was cross-linked with three different Jeffamines with functionality ranging from mono to trifunctionality. These curing agents were used to compare functional primary amine molecules. Epoxide-Amine coatings also have been studied at three temperatures ranging from 80 ◦C to 150 °C. The epoxide-amine cured systems were prepared with 1:1 epoxy:amine molar ratio at room temperature. Three epoxy networks based on epoxy functionalized tung oil (ETO), respectively, cured by Jeffamines were compared. The cured coatings were analyzed by IR, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and, gel content test. TGA analysis revealed that epoxide-amine polymers exhibited thermal stability up to 355 °C. DSC displays that the cured films have glass transition in the range of 83–189 °C. The amine cured epoxides were obtained with fairly high Tg values and excellent thermal stability. Mechanical performance properties of these films were investigated by pendulum hardness, pencil hardness, crosshatch adhesion, pull off adhesion, impact resistance and, reverse resistance. It has been observed that generally the epoxide-amine systems cured at 150 °C show very good pencil hardness (6H), pull-off adhesion (3->5 lb/in2), crosshatch adhesion (5B), impact resistance (>40), reverse resistance (>40), and chemical resistance (70-135 MEKDR) properties according to the ASTM standards

Biography

ILKNUR BABAHAN received Ph.D. degree in Organic Chemistry from Ege University, Izmir, Turkey in 2005. Since 2005, She has been with the Faculty of Arts and Sciences, Adnan Menderes University (Aydin, Turkey), where she is currently an Associate Professor. She went on to do postdoctoral work at The University of Akron (Akron, OH, USA) in 2012 and at Morgan State University (Baltimore, MD, USA) in 2015. Her main research interests include synthetic organic chemistry; design and synthesis of novel compounds which may be used as “smart medicine”; synthesis of polymeric resins (Alkyd/Epoxies/Acylates/Urethanes) and development of new UV curable materials.

Speaker
ILKNUR BABAHAN / Adnan Menderes University, turkey

Abstract

Sirtuin 1 (SIRT1) is a histone deacetylase (HDAC) from Sirtuin family. High expression of SIRT1 induces activation of DNA repairing factors eventually leading either tumor suppression or tumor development in cellular context dependent manner. Most of the chemotherapeutics induce apoptosis by creating DNA damage in tumor cells. However, SIRT1-activated repair of the chemotherapeutic-induced DNA damage results in decreased apoptosis and finally chemotherapy resistance. Therefore, inhibition of SIRT1 is a promising strategy both in anticancer therapy and preventing chemotherapy resistance. In this study, SIRT1 siRNA carrying liposomal formulations have been formulated by using N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) as cationic lipid by freeze drying method. Their effect on SIRT1 silencing has been determined as 2.5-4 folds both on mRNA and protein levels on prostate cancer cells (LNCaP, Du145 , and PC3) with different p53 expression pattern. Efficiency of the formulations to enhance the activity of doxorubicin, which is a chemotherapeutic performing its activity by creating DNA damage has been studied and found to increase the activity of doxorubicin on cell death although they have no effect on cell viability when they applied onto cells alone. Besides, enhanced DNA damage recognition after SIRT1 silencing by the formulations has been determined as the underlying cause of increased cell death. Further, no negative effect of the formulations on cell viability of normal prostate cells has been evaluated as a promising result for selectivity on cancer cells. As a conclusion, a series of siRNA carrying liposomal formulations for SIRT1 inhibition is able to enhance the chemotherapy efficiency in combinatorial use with DNA-damaging chemotherapeutics on prostate cancer cell lines.

Biography

Bilge Debelec Butuner has completed her PhD in 2012 from Ege University, Turkey and postdoctoral studies from Ege University, Faculty of Pharmacy. She has been working in the same department as Assistant Professor since 2016 with the focus on cancer therapeutics, drug delivery, cancer immunology and immunotherapy.

Speaker
Bilge Debelec Butuner / Ege University, Turkey

Abstract

A new multidentate ligands 1,1,2,2-tetrakis {(2-benzylthio) ethylthio} ethylene(L1) ; 1,1,2,2-tetrakis {(3-benzylthio)propylthio}ethylene (L2) ; 1,1,2,2-tetrakis {2-(o-tolylthio)ethylthio} ethylene (L3) ; 1,1,2,2-tetrakis {3-(o-tolylthio) propylthio} ethylene (L4) ; 1,1,2,2-tetrakis {2-(4-chloro-pheny-lthio) ethylthio} ethylene (L5) ; 1,1,2,2-tetrakis {3-(4-chloro-phenylthio) propylthio} ethylene (L6) ; 1,1,2,2-tetrakis {2-(o-aminophen-ylthio) ethy-lthio} ethylene (L7) ; 1,1,2,2-tetrakis {3-(o-aminophen-ylthio)propylthio} ethylene (L8) , were prepared from the reaction between 1,1,2,2-tetrachloroe-thlene and equimolar amounts of potassium hydroxides and 4-mercapto-2-thia-1-butyl benzene or 4-mercapto-2-thia-1-pentyl benzene or o-methyl-4-mercapto-1-thia-1-butyl benzene or o-methyl -4- mercapto-1- thia-1-pentyl benzene or p-chloro-4-mercapto-1-thia-1-butyl benzene or p-chloro-4-mercapto-1-thia-1-pentyl benzene or o-amino-4-mercapto-1-thia-1-butyl benzene or o-amino-4-mercapto-1-thia-1- pentylbenzene . Complexes of general formula [Au2(L)Cl4]Cl2 (L=L1-L8) were prepared through the direct reaction of the above ligands with H[AuCl4] in (1:2) ligand to metal molar ratio . The prepared complexes were characterized by their elemental analysis , IR , UV-Vis , spectra magnetic measurements and conductivity measurements .

Biography

will update soon...

Speaker
Anwer Alimam / University of Mosul , Mosul – IRAQ

Abstract

Slow volume fluctuations of liquids media, inside cranium with irregular amplitude and frequency are attracted attention of scientists for a number of decades, but their biophysical background and physiological significance, is unclear up to the .present time. Reason for this was the absence of adequate methods of their analysis, which should base on the use statistical approach. Goal of present investigations Is investigation of the possibility to the use spectral approach for slow intracranial volume fluctuations analysing. For this purpose intracranial volume liquid fluctuations was transformed to fluctuation electrical impedance, because electrical conductivity of blood-CSF and brain tissue on 100 kHz are significantly different. So, intracranial liquid fluctuations will correspond to head electrical impedance changes, which is possible to investigate by spectrum modern instrumentation. It was received, that spectral diagrams of slow fluctuation inside cranium are characterized by permanent fluctuations. It was established that most valuable are fluctuations of 0, 1 – 0, 3 Hz. Infrastructure of such diagram consists from 4 -7 peaks of spectral lines and with amplitude 0.4 –0.7 unit to compare with maximal pulse amplitude. Pattern of spectral diagram depends from brain activity, age and characterized by hemispheric asymmetry. Received data permits to conclude, that intracranial slow volume fluctuations are supporting also homeostasis of the working brain. Thus, wave volume fluctuation inside cranium cavity may be an informative method for diagnostic and for evaluation general status of patients as well as evaluations of mechanism of treatment basis of some neurological techniques in cranial field.

Biography

Moskalenko Yu. has completed his PhD from Institute medico-biological problems in Moscow, RF and postdoctoral studies in Burden neurological Institute, Bristol, UK. He is a director of Laboratory Cerebral circulation in Sechenov Institute Russian Academy of Sciences. Author more 180 publications in reputed journals and 7 books, covering different aspects of biophysics and physiology of brain blood and SCF circulation. Kravchenko T. has completed her PhD from Pavlov medical Institute in S-Petersburg, RF, and postdoctoral studies in Russian high school of osteopathic medicine in Paris,France. She is a Principal of Medical academy of osteopathic education. Autor more then 30 papers in reputed Journals, covering different aspects of osteopathic medicine in cranial field

Speaker
Yuri Moskalenko / sechenov indtitute of russion Academy

Plenary Talks

Abstract

Nitrous oxide (N2O) is known as a serious greenhouse gas and ozone depleting substance. N2O can only be consumed by denitrification, a pathway that only exists under anoxic condition1). However, the recently study has suggested that cyanobacteria with N2-fixation ability can fix N2O. Now, only three kinds of cyanobacteria are known as the N2O fixation cyanobacteria, and there is no report on the suitable conditions for the N2O fixation. Furthermore, the N2O fixation pathway has the two theory : (1)Fix N2O directly to the bacterial cell (2) Fix N2O after reducing to N2, which is clearly not clarified. The purposes of this study are to exploration of N2O-fixing cyanobacteria and clarify N2O fixation conditions & N2O fixation pathway. For exploration of the N2O fixation cyanobacteria, we focus on the report that a nitrogen fixing enzyme use N2O as a substrate and we hypothesized that the cyanobacteria with N2-fixation ability can also fix N2O. As a result of cultivating the N2-fixing cyanobacteria with 46N2O under the condition of He, 15N Atom% in the cells of Nostoc sp, significantly increased compared with the natural isotopic ratio of 15N (0.366%), suggesting the N2O fixation ability. Nostoc sp. ware cultivated with 46N2O under the different oxygen concentration. It was revealed that Nostoc sp. have N2O-fixing activity even under aerobic conditions which denitrifying bacteria can not reduce N2O. It was also shown that oxygen inhibit on the N2O fixation. As a result of cultivating Nostoc sp. with 46N2O under the condition of 95% N2, 46N2 0 was consumed at a rate of 40% compared with the condition of without N2 (= 95% He). Since 30N2 was generated during this cultivation, it was revealed that the N2O fixation pathway is via N2. About 40% of consumed N2O was fixed to the cell under the condition of 95% He. On the other hand, under the condition of 95% N2 concentration, most of consumed N2O was released as 30N2. This result suggest that N2O fixation also occurs universally under the conditions where N2 fixation occurs. It was revealed that N2O was fixed to the bacterial cells after reducing to N2.

Biography

hosomi masaaki is currently working as Faculty of Engineering Department of Chemical Engineering Graduate School of Engineering Department of Applied Chemistry his reasearch interest: Cleanup of Groundwater Contaminated with Chlorinated Organic Compounds by Persulfate Chemical Oxidation

Speaker
Masaaki Hosomi / Tokyo University of Agriculture and Technology, Japan

Sessions:

Bioscience& Biotechnology

Abstract

Heliconiaceae family only comprises one genus; this unique taxa correspond to Heliconia. This genus includes flowering plants with high economic interest, characterized by its bracts with intense colors. Notwithstanding, some phenomena related to hybridization, inbreeding and genetic drift have created a great natural variability between cultivars of the same specie, making classification activities difficult. Considering its interest within the exotic flower trade, this research aims the development of SSR markers proper of Heliconia bihai, H. caribaea, H. orthotricha y H. stricta species as tools to assess genetic diversity and to be able to stablish variety classification. Thus, 33 cultivars of described species, including a interspecific hybrid (H. caribaea x bihai), were sampled to its further genetic characterization. Every employed loci showed multi-allelic behavior on evaluated species with polymorphic index content (PIC) ranging within 0,403 and 0,821. In addition, species with minor genetic distance were H. caribaea and H. caribaea x bihai, and species with the greatest genetic distance were H. stricta and H. caribaea x bihai. As expected, the majority of evaluated markers showed high HWE deviation

Biography

Will update soon.....

Speaker
Marta Leonor Marulanda / Tecnológical de Pereira, Pereira, Colombia

Abstract

In the process of bacteriophage integration, the site-specific integrase recombinase (Int) encoded by the lambdoid coliphage HK022targets in the chromosome of its E. coli host a singleshort recombination site (attB) of only 21 base-pairs (bp) long. attB comprises two partially-inverted 7 bpInt-binding sites (B and B') that flank a central 7 bp crossover site known as the “overlap” (O), altogether BOB'. Int-HK022 has been recruited to function as a site-specific recombinase in human cells. Interestingly, Int-mediated site-specific recombination proceeds also when the 7 bps of O are replaced by a random 7 bp sequence provided that its cognate phage recombination partner attP features an identical O sequence. A search of the human genome has enabled us to identify native sequences matching such modified active attB sites (“attBs”) that flank deleterious human disease mutations. The existence of these “attB” sequences raises the prospect of curing human mutations they flank by an Int-HK022 catalyzed recombinase-mediated cassette exchange (RMCE) reactions. Experiments with mutations of human diseases demonstrate the feasibility of these reactions for human gene therapy.

Biography

Ezra Yagil has completed his PhD from the University of California at Davis, USA and postdoctoral studies at the Biology Department at Brown University, Providence Rhode Island, USA. At present he is Professor Emeritus at Tel-Aviv University Israel. His expertise is in bacteriophage genetics and the implementation of site-specific recombination systems in eukaryotic organisms

Speaker
Ezra Yagil / Tel-Aviv University, Israel

Abstract

The dynamic surface tension (DST) data and biochemical parameters (BCP) of animal blood have been obtained. The strong positive correlation was found for goats between σ1, σ2, σ3 (DST) and sodium levels (BCP), λz (DST) - with the levels of lipid and sodium (BCP); whereas strong negative correlation was found for goats between σ3 (DST) and the level of total protein and chloride (BCP), λz (DST) – with the level of albumin (BCP). The strong positive correlation was found for horses between σ1, σ2, σ3 (DST) and lipid levels (BCP); λ0 (DST) - the level of chloride (BCP); λz (DST) - the level of albumin and chloride (BCP). The strong negative correlation was found for horses between σ1 (DST) and sodium level (BCP); σ2, σ3 (DST) and chloride level (BCP); λ0 (DST) and lipid level (BCP). Some middle and weak correlations of different types were also found, but that had less importance for practical usage [1,2]. The particular correlations between BCP and DST data of the same serum samples allowed to check only DST data directly at farm and use more expensive and time-consuming biochemical analysis only by high necessity for particular animal. 1. Zaitsev S. Yu. Dynamic surface tension measurements as general approach to the analysis of animal blood plasma and serum. Advances in Colloid and Interface Science, 2016, 235, 201–213. 2. Zaitsev S.Y. Tensiometry and biochemical analysis of the blood of animals: fundamental and applied aspects; "Agricultural Technology" Publishing House: Moscow, Russia, 2016.

Biography

Sergey Yu. Zaitsev graduated from the Department of Chemistry of Moscow State University in 1980 and worked at different positions (from a junior research assistant to an acting director of laboratory) in the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow. Since 1999, he has been working as the head of the Chemistry Department of the Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology (Moscow SAVMB). He has Doctor of Science degree in chemistry (1995) and biology (2007), a professorship at the Moscow SAVMB (2005). He has published about 150 papers in reputed journals.

Speaker
Sergei Yu. Zaitsev / Moscow,Akad. Skryabin Str. Russia

Abstract

Following the seeming insurmountable medical cure for the dreaded type-II diabetes, several and concurrent notable scientific research works for the most appropriate approach for the treatment and management of the aforementioned disease have been on the increase. In this paper, using ordinary differential equations, we formulated a set of pent-linear mathematical type-II diabetes dynamic model. The novelty of investigation was primed by a tri-linear optimal maximization of model predominant state variables following methodological application of designated bilinear control functions in the presence of incorporated time delay lag. With derived model, the system invariant and boundedness of solutions as well as stability analysis was scientifically conducted. To achieve study set goal, the model was transformed to an optimal control problem and analysis performed using classical Pontryagin’s maximum principle. The system optimal characterization, existence of an optimal control pair and optimality system were comprehensively established. The numerical result that follows conspicuously showed the pragmatic flow of the model as evidenced by the highly tri-linear maximization and sequence reversion of type-II diabetes’ early infection stages. Moreso, the near zero reduction of chronic type-II diabetes infection was a further affirmation of model ingenuity, which is a step towards achieving the much needed bioscientific and biotechnological height sought for this 21st century. Suggested therefore, is a more chemotherapy inclusive and possible extensively articulated method for a possible eradication of this dreaded type-II diabetes.

Biography

Bassey Echeng Bassey, a philosopher in Computational Mathematics and Informatics from Kuban State University, Krasnodar Russia and M.Sc. in Industrial Mathematics, Federal University of Agriculture, Makurdi, Nigeria. He is a senior researcher/lecturer and presently the Co-coordinator – Head of Pre-degree program, Cross River University of Technology, Calabar, Nigeria. Published 28 papers in reputed journals including 3 recent published articles in 2018, monographs’ and authorof textbooks with citation of over 217 times and 9 h-index. Serving as an editorial board member and peer-reviewed of number of reputed journals. Research interests include but not limited to: Mathematical modeling, numerical methods; simulation analysis, mathematical methods, computer technology and programming

Speaker
Bassey Echeng Bassey / Cross River University of Technology,Nigeria

Abstract

CBP and p300 are histone acetyltransferase coactivators that control the transcription of numerous genes in humans, viruses, and other organisms. Although two separate genes encode CBP and p300, they share a 61% sequence identity, and they are often mentioned together as p300/CBP. Zhou et al. showed that under hypoxic conditions, HIF1α and the tumor suppressor p53 compete for binding to the limiting p300/CBP coactivator. Jethanandani & Kramer showed that δEF1 and MYOD genes compete for the limited amount of p300/CBP in the cell. Bhattacharyya et al. showed that the limiting availability of p300/CBP in the cell serves as a checkpoint for HIF1α activity. Here, we use the microcompetition model to explain how latent viruses with a specific viral cis-regulatory element in their promoter/enhancer can disrupt this competition, causing diseases such as cancer, diabetes, atherosclerosis, and obesity.

Biography

Dr. Polansky is the director of the Center for the Biology of Chronic Disease (CBCD). Dr. Polansky received his PhD on Computational Models of Intuition from the Hebrew University in Israel. He then worked at Cornell University, and the University of Rochester. Dr. Polansky wrote the book: Microcompetition with Foreign DNA and the Origin of Chronic Disease. Dr. Polansky is also the developer of the psycholinguistic-based data-mining program called Computer Intuition. The program uncovers the intuition of scientists hiding in scientific papers. Dr. Polansky is using Computer Intuition in in uncovering surprising relationships hiding in the scientific literature.

Speaker
Hanan Polansky / The Center for the Biology of Chronic Disease, USA

Abstract

The adoption of a biological method of plant nutrition has proved to be extremely efficient. The method does not involve the use of chemical salts as used in traditional plant fertilizers. These fertilizers used over some 80 years has had a deleterious effect on soil health due to the diffusion of the salts used which often lead to over application. The new approach to improvesoil health and plant growth uses natural crushed zeolite rock and organic waste as a soil amendment. This material supplies nitrogen, phosphorus and potassium together with minor and trace elements which are essential and beneficial to plant growth. Zeolite minerals are well known to adsorb ammonium ions which are provided by the decomposition of the organic component. When the organo-zeolitic mixture is added to a soil the ammonium ions arereplaced by soil Potassium . The slow release of the ammonium ions are oxidised by Crenarcheota, a soil micro-organism that is now known to be the most prevalent ammonium oxidizing in the soil environment. The behaviour provides a gradual accumulation of nitrate in an ionic condition that can be accepted by the growing plant. In this way there is very little access nitrate to diffuse into the soil and with phosphorus supplied from the organic waste and potassium, also available, the three major elements (NPK) are present.As the process of nitrification , involves enzymes which produce hydrogen in the form of hydronium ions that arevery reactive releasing a range of elements from the soil which provide minor element ions in trace quantities required for strong plant growth.The organic component maintains an adequate supply of carbon which is lost in the application and long-term use of traditional chemical fertilizers. The presence of soil carbon is essential for soil health as its reduction results in loss of soil structure and water holding capacity, resulting in a fragile soil which is prone to transportation by wind and rain. The dust bowls of the Midwest of the USA are a spectacular example of this effect. Thus the adoption of the use of the organo-zeolitic-soil system, in countries that benefit from presence of economic deposits of zeolitic tuff, is a step forward in arable farming and the phytoremediation of contaminated land as well as the production of fuel crops on such land and the improvement of marginal farmland.

Biography

will update soon

Speaker
Peter J. Leggo / University of cambridge UK

Abstract

In agronomy, the release of sequenced genomes allows the identification of gene families whose protein products may be of interest for molecular breeding and the development of improved varieties. The relief of reference genomes, the progress in genomic resources and the availability of increasingly complex bioinformatics tools can contribute to this advance. Thus, several gene families in plant species have recently been identified and characterized. Bioinformatics is currently a fundamental branch in genomic studies. This work has been based on the systematic search of sequences in the data bank (GeneBank, NCBI). For this we have applied a series of scripts written in the programming language R in order to reduce manual steps as much as possible and thus be able to extract, filter and catalog the search results in an efficient way. The codes for this work have been written in the R programming language, with the R software and the free access RStudio. Without the free access to the databases information, the work would not have been possible. Therefore, this work highlights the importance of open science. According to this, all the scripts elaborated for the data analysis, together with the functions documentation, as well as the main results, are freely available to the community so that they can use them and adapt them to their projects

Biography

Carmona-Molero, R., Die, J.V.. Millán, T. Identification and Characterisation of Aldehyde Dehydrogenase Gene Superfamily in Cicer arietinum through open access bioinformatic tools. Universidad de Córdoba, Bioinformatics, Publication will have place around March 2019

Speaker
Rocio Carmona Molero / University of Córdoba, Spain

Abstract

An experiment was carried out in the laboratories of Date palm micropropagation Unit at the College of Agriculture, University of Kufa during the period from August 2017 to June 2018 to study the impact of plant growth regulators and light quality on propagate banana(Musa spp.) CV. Dwarf Cavendish (using plant tissue culture technique, BAP (3 ,4 ,5 ,6 mg.L-1) and TDZ ( 0.1, 0.2, 0.3 mg.L-1) alone or incombination with NAA ( 0.5 ,1 , 1.5 mg.L-1) and light sources, Florescent white and LED light ( 18 red :2 blue ),on shoot multiplication was studied. Shoot cultured on MS with 0.1 TDZ + 1.5 NAA under LED light gave highest values for shoot number and length, chlorophyll and carotin contents ( 9.25 shoot, 4.12 cm, 50.18 mg/100g FW and 5.070 mg/100g FW ) respectivity ,but shoot on MS with 0.2 TDZ + 1.5 mg NAA under LED light gave highe shoot fresh and dry weight and shoot containt of carbohydrate which gave 20.28 g ,0.6925 g and 2.917 respectivty.

Biography

will update soon

Speaker
Siham A. Salim / Al-Furat Al-Awsat Technical University Iraq

Will be updated soon...