High-viscosity injector-based Pink Beam Serial Crystallography of Micro-crystals at a Synchrotron Radiation Source
Wei Liu joined the School of Molecular Sciences and the Biodesign Institute at ASU in 2015. He came to ASU from the Scripps Research Institute and The Ohio State University, where he spent over a decade developing new tools for studying the structure and function of membrane proteins with a focus on GPCRs involved in cancer.
Professor Liu earned his doctorate in biophysics from The Ohio State University in 2007 under the mentorship of Professor Martin Caffrey, which ignited his passion for studying membrane proteins and developing lipidic cubic phase (LCP) technology. He then joined the lab of Professor Raymond Stevens and Professor Vadim Cherezov at the Scripps Research Institute where he played a critical role in solving the first high-resolution structure of human adenosine A2A receptor with a novel fusion protein (BRIL) at 1.8 angstrom resolution, by far the highest resolution of all human membrane protein structures and was highlighted by Science Magazine. The scientific insights and technical advances from this initial work were used by Professor Liu and colleagues to solve the structures of 10 additional unique human GPCRs. These structures have enhanced our understanding of how GPCR folding has evolved to bind such a large and diverse pool of natural ligands.
More recently, Professor Liu has applied his LCP crystallization methods for studying GPCRs using X-ray free electron lasers (XFEL), which does not require sample freezing, has the potential to minimize radiation damage (if any) during data collection due to the ultrashort (femtosecond) pulse duration, and only requires relatively small crystals making it much more accessible for many GPCRs recalcitrant to crystallization. He solved the first human membrane protein and GPCR structure using XFEL, the human serotonin 5-HT2B receptor. His growing body of work now encompasses over 40 peer reviewed publications, including 16 in Nature, Science and Cell journals with over 6000 citations.
Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow, showing that 3rd generation synchrotrons can be viable alternatives to scarce X-ray free electron laser sources. Synchrotron radiation flux may be increased about 100 times by a moderate increase in bandwidth (“pink beam” conditions) at some cost in data analysis complexity. Here, we report the first high-viscosity injector-based pink beam SX experiments. The structures of A2A adenosine receptor (A2AAR) and proteinase K (PK) were determined to 4.2 Å and 1.8 Å resolution using 24 and 4 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A2AAR required an alternative data processing strategy. This demonstration of the feasibility presents new opportunities for the time-resolved experiments with micro-crystals to study structural changes in real-time at pink beam synchrotron beamlines worldwide.
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Dr. Wei Liu has completed his PhD from The Ohio State University, USA and postdoctoral studies from The Scripps Research Institute, USA. He is now a faculty member at Arizona State University. He has published more than 50 papers in reputed journals and has been serving as an editorial board member of repute.
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Dr. Vladimir Tolstikov was awarded with PhD degree at the age of 28 years from the Institute of Chemical Means for Plant Protection, Moscow, Russia. During his career he was working in leading research academic institutions and industry including research institutes of Russian Academy of Medical Sciences, Max Plank Institute, UC Davis, Eli Lilly and Company and others. He is a pioneer in HILIC separations development applied to Metabolomics. He is the director of Metabolomics at BERG LLC. He has published more than 60 papers in reputed journals, 5 book chapters and has been serving as an editorial board member of MDPI Metabolites. He has participated and contributed in organization of various international meetings. His current research interests are in the field of AI-guided multi-omics input into the Precision Medicine development.
• 1989-1996 Department of Medicine, School of Medicine, Chang Gung University
• 2014 Research award of clinical medicine. Taipei Medical University, March, 2015.
• Inclusion in Who’s Who in Medicine and Healthcare 2011-2012 (8th edition)
• Inclusion in Who’s Who in the World, 2011, 28th edition
• The Second Best Chart Record in April, Department of Internal Medicine, Chang Gung Memorial Hospital at Keelung, 2001.
• 3/2011-Present Assistant Professor of Medicine, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University
• 7/2008-Present Attending Cardiologist, Division of Cardiology Shuang Ho Hospital, Taipei, Taipei Medical University
• 7/2003-5/2008 Attending Cardiologist Division of Cardiology Chang Gung Memorial Hospital, Taoyuan
• 7/2001-6/2003 Cardiology Fellowship 1-2, Division of Cardiology Chang Gung Memorial Hospital, Taoyuan
• 7/1998- 6/2001 Residency 1-3, Internal Medicine, Chang Gung Memorial Hospital, Taoyuan
• Dr. Hung’s researchinterests are focused on C-reactive protein in the dynamic coronary stenosis. His laboratory attempts to apply basic and biostatistical findings to the possible pathophysiologic mechanism in coronary spasm (dynamic coronary stenosis). In addition, Dr. Hung is also interested in left ventricular diastolic function evaluation using echocardiography. His echocardiographic laboratory attempts to examine the left ventricular anatomical and functional changes in patients with coronary spasm. Currently, Dr. Hung is studying the left ventricular changes using echocardiography in a retrospective study for patients with coronary spasm. Summary of ongoing projects in his laboratory include:
• Coronary intervention, Peripheral intervention (hemodialysis AV shunt angioplasty), Echocardiography
• Investigating the mechanisms by which inflammatory cytokines play in the mechanism of coronary spasm.
• Investigating the left ventricular anatomical and functional changes in patients with coronary spasm.
Jaleel Kareem Ahmed has expertise in evaluation in Iron and steel industry . He registered 3 patents in USA , UK and Iraq about using water in iron industry and wax for storage and transportation of Direct Reduced Iron (DRI) , and using wax for carburizing of steel. Also he used chlorophyll as gamma ray absorber to protect Iraqi children from cancer . He used the mechanically red beet juice as scavenger for poisonous heavy metal ions and anticancer and detoxification of urea and uric acid from human body via urine system . In 2013 he awarded Scientific Medal from Iraqi Government . In 2014 he qualified as a member in Who is Who network. He has been Serving as a reviewer of Journal of Advances in Polymer Technology / Thomson Reuters . He take part in 2016 & 2017 for Quality Star (QS ) World University Ranking Supplement for the QS Intelligence Unit .
Dr. Rahul Hajare is bright student of Renowned Immunologist Honorable Respected Dr. Ramesh S Paranjape’’ Retired Director and Apex body member of Indian Council of Medical Research New Delhi. He is Fellow Indian Council of Medical Research. He is PhD student of reputed University Vinayaka Mission Research Foundation Salem TamilNadu. He is winner of world academic championship-2017 in pharmacy (antiretroviral therapy). He is among world's 500 most influential experts in pharmacy for the year 2017 on earth. He is alumni of The Yoga Institute Centenary Celebration Mumbai.