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Poster Presentations

Abstract

The aim of this study was to prepare the immediate release tablets from hot melt extruded Soluplusbased solid dispersion of poorly water soluble drug, Celecoxib. Three types of hot melt extruded solid dispersions were prepared with the ratios of drug and carrier 1:1(SD-1), 1:3(SD-2), and 1:5(SD-3). Solupluswas blended with celecoxib by hand mixing and then extruded through a co-rotating twin-screw extruder (11mm Process, ThermoFisher Scientific) at 160°C and 100rpm. To check quantitative analysis, the dissolution test was performed using the USP apparatus 2 (paddle) method. SEM, DSC, PXRD and Raman spectroscopy were performed to understand the physico-chemical properites. The content test of all hot melt extruded solid dispersions were approximately 97%, drug release on FN-1 tablet showed similar behavior with marketed drug, Celebrex. Furthermore, FN-2 and FN-3 demonstrated 40~50% of higher drug release behaviors. FN-2 reached 79±3.84% in distilled water, 75±4.50% in simulated gastric fluid, and 85±2.90% in simulated intestinal fluid within 120min. FN-3 showed a dissolution rate of 83±4.29% in distilled water, 90±4.55% in simulated gastric fluid, and 89±2.54% in simulated intestinal fluid. Based on SEM, DSC, PXRD and Raman spectroscopic analysis, celecoxib and physical mixture were crystalline, while Soluplusand solid dispersion were changed to amorphous status. Consequently, drug release patterns were greatly enhanced as compared with the marketed drug. Acknowledgement : This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIP) (No. 2017R1C1B1003590).

Biography

Jun-Bom Park has completed his PhD from College of Pharmacy, Kangwon National University, Republic of Korea and postdoctoral studies from Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, USA. He has published more than 50 papers in reputed journals.

Speaker
Jun-Bom Park / Sahmyook University
Korea (South)

Abstract

N-heterocyclic carbenes (NHCs) are an essential class of ligands in the rich organometallic chemistry of low-valent late transition metals, playing a vital role as possible metal-based drugs and a great variety of catalysts in the reactions including C–C couplings, C–H activation and hydrosilylation. After a significant period of exploration into the organometallic chemistry of gold/silver complexes that started in the mid-1990s and crowned with the establishment of a variety of functionalized and non-functionalized derivatives as efficient biologically relevant and NHC transfer candidates. Particularly, various investigations focused on the role of gold/silver–NHC complexes as metal-based drugs towards human derived renal, colon/colorectal and breast cancers. In this study, the synthesis, structural analysis, and bioactivity of silver-NHC and gold-NHC complexes bearing a coordinating anion were performed and assessed. Two series of chloride and non-chloride Ag-NHC-acetate and Au-NHC-acetate were prepared. The gold complexes were afforded by trans-metallation. Additionally, the complexes and their pro-ligands have been fully characterized by NMR and mass spectroscopy analysis. The compounds have been evaluated for their antimicrobial activity for a sample panel of 24 bacterial strains, performing the Agar Well Diffusion assay. The data showed activity for the gold series, whilst the silver complexes displayed a non-satisfactory activity.

Biography

Dr. Maria Grazia Bonomo is a biologist and holds a class honours degree in Biological Sciences and has completed her PhD in Biology and Biotechnologies from University of Basilicata, Italy. She is a professor of teaching ‘Physiology and Genetics of Microorganisms’ for the Degree Course in Biotechnologies and she has a post-doc fellowship at University of Basilicata and performs supplementary teaching activities for the course of General and Applied Microbiology. She is author/co-author of many international papers and an editorial board member and/or reviewer of reputed journals.

Speaker
Maria Grazia Bonomo / University of Basilicata
Italy

Abstract

Haemoglobin-based oxygen carriers (HBOCs) are being developed as substitutes to replace the oxygen-carrying functions of erythrocytes and thereby lessen the demand of donor blood during surgery and trauma situation (Chang, 2000). The HBOCs are designed to increase the oxygen-carrying capacity while reducing the risk commonly associated with allogenic RBCs transfusion ( Kim and Greenburg, 2006). Bovine and human haemoglobin form the bases of many different types of HBOCs (Jahr et al., 2008; Chen et al., 2009; Taguchi et al.,2010). The aim of this study was to develop an effective and safe acellular HBOC blood substitute. The study comprised 2 stages: Stage I : Laboratory preparation of camel haemoglobin (CHBOC) Stage II: Experimental application of the developed CHBOC on 10 normal adult dogs. The dogs were randomized into 2 groups (Test=7 dogs-14 trials and Control=3 dogs-6 trials). Both groups were subjected to exsanguination of 40% of estimated blood volume to achieve severe hypovolemia. The test group was infused with CHBOC (40 gm/L) dissolved in lactated Ringer's. The control group was infused with HES 200 (6gm/L) (Hydroxyethyl starch) dissolved in Saline. Blood samples were collected from the dogs of both groups at base line before exsanguination (BE) and after exsanguination (AE)by one and 24 hours for CBC and estimation of certain blood biochemical values reflecting the liver and kidney functions. The clinical, haematological and biochemical responses were normal. The CHBOC showed improved early survival and stabilized physiological and haemodynamic functions. Survival was not altered in the experimental dogs of both groups. It is interesting to note that, administration of CHBOC to the test group was associated with remarkable vitality , a high capacity for exercise and a great appetite for up to 2 days post-infusion. On the contrary, the control group infused with HES 200 solution exhibited weakness and reduced appetite for nearly one weekpost-infusion. The recorded changes in certain physiological variables after exsanguinations ( AE) and 1 hour post infusion (1hA) were slight hypothermia and marked tachycardia in both groups . Blood biochemistry parameters reflecting renal function (BUN and creatinine) and liver function (bilirubin, AST and ALT) showed minor changes post-infusion from the values at base line . Other parameters reflecting metabolic activities (glucose, cholesterol, triglycerides and albumin) showed significant elevation of the glucose value (P<0.05) in the test group received CHBOC 1hr-post-infusion.

Biography

Mohamed Mostafa Shokry has completed his PhD from Cairo University, Egypt. He is a Professor Emeritus of Veterinary Surgery & Anesthesia in Cairo University, Egypt. He has published more than 75 papers in scientific journals and has published many library books.

Speaker
Mohamed Shokry / Cairo University
Egypt

Abstract

Genus Micromeria Bentham belongs to the Lamiaceae family. Micromeria frivaldszkyanais a perennial herbaceous plant – a rare and endemic species, inhabiting rocky regions in the Central and Eastern Balkan Mountains in Bulgaria.Phytochemical studies of rare and endemic plants with specific habitat are scientifically interesting and thus the assessment of the biological features, spread and natural resources of Micromeria species is important for their conservation and sustainable use. The methods of measuring of antioxidant activity vary and they are based on generating radicals acting through different mechanisms. The current study is based on the electrochemical method, which measures the electrical signal derived from the oxidation of the substance isolated from the plant extract (or mixture), that is tested for antioxidant activity (AOA), on the surface of a working electrode at a given voltage. This method determines the overall antioxidant potential of a sample. Aqueous, methanol and ethanol extracts (96% and 70%) are prepared from the dry extract of Micromeria frivaldszkyana. Concentraions of 100 µg/ml of each were used. The antioxidant activity was measured electrochemically by determining the kinetic criterion K (the amount of oxygen forms reacting with the sample over time), the values of which, along with the AOA, are calculated against that of Trolox.It was found that the highest K belongs to the 70% ethanolic extract of the plant MF - 44.109±2.865(μmol/l.min±SD), as opposed to the one of Trolox - 3.619±0.013,exhibiting 12.188 times higher AOA. In compaison with it, water extract has K 12.905±0.843 and AOA is 3.566 times higher than trolox.

Biography

Dr.Anelia Bivolarska graduated and completed her PhD at the Medical University of Plovdiv, Bulgaria. She is an Associated Professor in the Faculty of Pharmacy at the Department of Chemistry and Biochemistry. She has published over 20 papers in reputed journals, monographs, textbooks and teaching aids.

Speaker
Anelia Bivolarska / Medical University of Plovdiv
Bulgaria

Abstract

Scutellaria species are known for their therapeutic properties due to the biologically active compounds contained therein.The flavonoids (scutellarin, baicalin, baicalein, wogonin, wogonoside, luteolin, chrysin) and verbascoside werequantified in water, methanolic and ethanolic extracts of Scutellariaaltissima, Scutellariaalbida, Scutellariagalericulata andScutellariacolumnae, the species wild growing in the different regions of south Bulgaria. So far, only diterpenecompounds and their antifeedant activity have been proven in these species. The aim of this study was to determine the flavonoid composition of Bulgarian speciesas a first step towards understanding their potential as a resource for pharmacologically active compounds.The content of typical for Scutellaria genus flavonoids was within very wide limits.Baicalin was found from 114 to 31250µg/g dry plant material, scutellarin – from 88 to 4350 µg/g, wogonoside – from 40 to 3429 µg/g. The best results were achieved with 70% ethanolic extraction. Verbascoside (caffeoylphenylethanoid glycoside) was found in methanolic extract only (147-1338 µg/g). The antioxidant activity of all extracts was done though measuring the Oxygen Radical Absorbance Capacity (ORAC), Hyrdoxyl Radical Averting Capacity (HORAC) and via electrochemical method (EM).

Biography

Stela Dimitrova has completed her PhD from Medical University of Plovdiv, Bulgaria. She is anAssociateProfessor of bioorganic chemistry inthe Department of Chemistry and Biochemistry. She has published more than 20 papers in reputed journals on the fields of bioorganic chemistry, biotechnology, drug delivery systems and pharmacology.

Speaker
Stela Dimitrova / Medical University of Plovdiv
Bulgaria

Abstract

The aim of this study was to prolong the antidiabetic action ofglimepiride to improve the patient convenience and compliance. A depot in situ gel formulation was designed and the main factorsthat control the initial burst and sustain the GMD effect was investigatedusing Box-Behnken experimental design. These factors were PLGA percent (X1), PEG percent (X2) and benzyl benzoate percent in N-methyl-2-pyrrolidone (X3). The results revealed that PEG percent is the only factor that showed significant effects on all investigated responses. In addition, PEG percent improved the smoothness and reduced the porosity of the ISG formulation surfacethat confirmed by scanning electron microscopy images. The resultsalso revealed that the optimized GMD-ISG formula (10 mg/ kg) does not exceed the maximum safe plasma concentration when compared with the oral marketed GMD tablets (1 mg/kg).Moreover, the optimized GMD-ISG formulation showed a depot effect that lasted for 14 days post-injection. This approach to controlling GMD release using an in situ forming system could be useful for improving patient compliance and diabetes treatment effectiveness.

Biography

Khalid Mohamed El-Say is working as a Professor in Department of Pharmaceutics,Faculty of Pharmacy, King Abdulaziz University, Saudia Arabia.

Speaker
Khalid Mohamed El-Say / King Abdulaziz University
Saudi Arabia

Abstract

The daily dynamics of intragastric pH in patients with acid-related diseases are different in comparison to healthy subjects of bioequivalence studies. The two most frequent causes are pathologic duodenogastric reflux (PDGR) in gastroenterological patients and multiple proton pump inhibitors (PPI) dosing. In the first case, rising pH≥7 asociated with pancreatic NaHCO3 secretion changes to pH decreasing that is closely related to intensive gastric acid production. In the second case, multiple PPIs dosing in patients with acid-related diseases leads to the pH increasing ≥4. Such variations are resulted in changing conditions of enteric-coated acid-labile drugs release and absorption. In view of this pathologic pH changes, we modify a comparative dissolution test (CDT) for PPIs. CDT (рН 1.2±0.05; рН 4.0±0.05, рН 7.0±0.05) has been used to estimate influence of PDGR and pharmacological acid suppression on omeprazole and rabeprazole. The additional aliquots as markers of PDGR-influence were taken from the рН 7.0±0.05 solution after 4 minutes for original omeprazole (OO) and generics (GO1, GO2, GO3) and 12 minutes for rabeprazole (OR, GR1, GR2). PDGR resistance for PPIs capsules and tablets was determined by active substances presence or absence. PDGR model demonstrated poor reflux influence on OO and GR1, strong influence on GO2 and GO3 with the almost complete omeprazole release and its potential degradation after following pH decrease. Pharmacological acid suppression model had demonstrated ОО, OR, GO1, GO3 and GR1 resistance while total GO2 and partial GR2 degradations were determined. Thus, proven pharmaceutical equivalence does not indicate therapeutic one.

Biography

Daria Kurguzova has completed a residency in Internal Diseases and Clinical Pharmacology at Sechenov University, Russia. Now she is completing her PhD dissertation in Clinical Pharmacology. She is the Assistant Professor at the Clinical Pharmacology Department and the Chief Specialist in Clinical Trials at the Department of New Drugs Research and Evaluation. She has published more than 25 papers in reputed journals and international conferences.

Speaker
Daria Kurguzova / I.M.Sechenov First Moscow State Medical University
Russian Federation

Abstract

A preliminary pharmacognostical and phytochemical evaluation of Smilax domingensis Willd., (Smilacaceae) was realized to determine the macromorphology and micromorphology characteristics, the physicochemical and phytochemical parameters from the rhizome of this spice that grows in Cuba. This crude drug showed the characteristic physicochemical values such as moisture content (13.11%), extractable matter in ethanol at 70% (13.53%), total ashes (3.45%), water soluble ashes (2.43%) and acid insoluble ashes (0.64%). Phytochemical screening revealed the possible presence of alkaloids, oils and/or fats, coumarins, saponins, flavonoids, pyrogallol-type tannins, quinones, catechins, reductants sugars, triterpens and steroids and absence of resins, aminoacids, cardiotonic glycosides, anthocyanidins and astringent and/or bitter principles, realized under WHO parameters. A preliminary chemical characterization of main components of ethanolic extract with dried rhizomes of S. domingensis that grow in Cuba was done using a GCMS-QP2010 Ultra Shimadzu and the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. After sample derivatization 125 chemical compounds were registered by the equipment and from them, 35 different chemical components were characterized and reported for the first time from this part of the plant in our country. Catechins were detected at 39.735 min after magnifying the area around that retention time. The results demonstrate the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Smilax domingensis.

Biography

A preliminary pharmacognostical and phytochemical evaluation of Smilax domingensis Willd., (Smilacaceae) was realized to determine the macromorphology and micromorphology characteristics, the physicochemical and phytochemical parameters from the rhizome of this spice that grows in Cuba. This crude drug showed the characteristic physicochemical values such as moisture content (13.11%), extractable matter in ethanol at 70% (13.53%), total ashes (3.45%), water soluble ashes (2.43%) and acid insoluble ashes (0.64%). Phytochemical screening revealed the possible presence of alkaloids, oils and/or fats, coumarins, saponins, flavonoids, pyrogallol-type tannins, quinones, catechins, reductants sugars, triterpens and steroids and absence of resins, aminoacids, cardiotonic glycosides, anthocyanidins and astringent and/or bitter principles, realized under WHO parameters. A preliminary chemical characterization of main components of ethanolic extract with dried rhizomes of S. domingensis that grow in Cuba was done using a GCMS-QP2010 Ultra Shimadzu and the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. After sample derivatization 125 chemical compounds were registered by the equipment and from them, 35 different chemical components were characterized and reported for the first time from this part of the plant in our country. Catechins were detected at 39.735 min after magnifying the area around that retention time. The results demonstrate the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Smilax domingensis.

Speaker
José González Yaque / Havana University
Cuba

Abstract

Introduction: The kernelpowder of the fruit of Balanites aegyptiaca(L.) Del is mixed with sorghum flour for the treatment of intestinal worms in Burkina Faso. Previous pre-clinical studies have demonstrated the efficacy and safety of the medicine. Objective: The objective of this study is to execute galenic formulations based on extracts of the kernel of the fruit of Balanites aegyptiaca(L.) with good pharmaceutical quality. Methods: The quality control wascarried out for the freeze-driedextracts of kernels and thesyrupswerepreparedthereon. Thin layer chromatography has been used to characterize thechemicalconstituents. Syrupswerepreparedfromlyophilizedaqueousextracts. Fifteen (15) testformulationswereprepared and the quality control of thesesampleshelped to sort out only one best amongthem. Results and Discussion: The results show that the syrupshad a good appearancewithoutsedimentation, itwaseasy to disperse and, itshowed absence of Salmonella and coliforms. Stabilitystudiesshowedthataftertwoyears’ of storage, the syrupskept the samecolors, taste andwere free of mold. The chemical composition of the syrupwassimilarduring the studiedstoragetime. Assessments of bacterial contamination of syrupsshowed an absence of Salmonella andcoliforms. After twoyears of storagethey have maintaineda good microbiologicalquality. Conclusion: This study made it possible to ensure good quality of the syrupsprepared. Parametersstudiedcanbeusedroutinely as elements of evaluation and quality control

Biography

Will be updated soon...

Speaker
Ouédraogo Salfo / Laboratory of Drug Development(LADME)
Burkina Faso.

Abstract

Biodegradable microspheres may develop improved drug delivery system to gastrointestinal tract, for treatment of Diabetes. Metformin hydrochloride have the ability to produce thus effect for extended period. The Objective of this Work to prepare metformin hydrochloride microspheres by using ethyl cellulose and polyvinyl alcohol as the retardant material with entrapment efficiency and extended release using solvent evaporation techniques. Also, the microspheres were prepared by the double emulsification technique with a yield (40%) are characterized . The Characterization was included : Optical microscopy, Scanning electron microscopy (SEM), Study the compatibility between the selected polymer and metformin hydrochloride by using IR and study the interaction between drug and polymer as well as the microspheres-drug polymer thermal behaviour and crystallinity by using DSC and XRD respectively. Drug release studies were conducted using the dissolution apparatus, the dissolution medium was phosphate buffer at pH 6.8. However the release of loaded metformin hydrochloride in microspheres occurred gradually of 30% up to 8 hours compared to the control which completely released within two hours.

Biography

Will be updated Soon...

Speaker
Abdulrhman Akasha / Tripoli University
Libya

Abstract

Introduction: Nanotechnological approach is significantly important in drug delivery system as nano- particles hold immense potential as a possible drug delivery system (DDS). In this review, we will discuss about the recent development of nanotechnology in DDSs. Nanotechnology in recent years have gained lot of significance because it can able to overcome the complications that are caused by gene and drug delivery. The toxic effect of the drugs on the normal cells can be greatly decreased. Therefore, this are the reasons why we use nanotechnology in drug delivery system. Using nanomaterials anti-cancer drugs like doxorubicin and loperamide that have successfully crossed the blood brain barrier and released into the brain. Nanoparticles take part in the crucial role as they can conjugate with wide variety of the drug molecules to deliver the active ingredient of the drugs to the respected target site. The surface of the nanoparticles is designed in a way to get affinity in the direction of distinct cells in the body and get protection from immune cells. They become temporally united with the drug and to join the target and enters the cell by the receptor-mediated endocytosis. Once the nanoparticles enter into the specific site, they will release the drug controllably and heal the disease. Methods: The review of the articles (54) on nanotechnological approach in drug delivery is given. The investigation is centred in India and Estonia. Results: From this review, we can study the start of nanotechnology, types of nanoparticles, classification of nanomaterials, nanotechnology uses in medicine, drug delivery system, mechanism of drug delivery using nanoparticles, nanotechnology uses in treatments.

Biography

S.Indukuru is the Master of Science student in Health Care technology in Tallinn University of Technology. He has the Bac degree in Pharmaceutics. P.Tint is the Prof of Occupational Health in TTU from 2000. Her PhD is from Leningrad Technological Institute from 1977 in chemical engineering. She has written over 200 scientific papers and is in the editorial boardsof 10 scientific journals over the world. She has successfully supervised 6 PhD students At the moment she is the programme manager for health care technology students in the infotechnology department in the speciality of occupational health and ergonomics.

Speaker
Sandeep Indukuru / Tallinn University of Technology
Estonia

Abstract

Pharmacognostic and physicochemical evaluations of the medicinal plants in herbal medicines and plant-based drugs formulation are the critical stage for validation of the medicinal plants quality. These analytical methods must be done according to reference protocols of World Health Organization monographs and pharmacopeia books in pharmaceutical industries. The Colchicaceae family have important alkaloidal compounds such as colchicine, demecolcine and cornigerin with high potential toxicity and narrow therapeutic index, which are used in some diseases such as gout and cancers. Pharmacognostic and physicochemical properties of some species of Colchicum including macroscopic and organoleptic properties, solubility, foreign matter, acid insoluble ash, sulphated ash, water soluble and water insoluble ash, heavy metals and total alkaloid have been evaluated. The pharmacognostic and physicochemical properties of C. speciosum, C. kurdicum and C. robustum corms are appropriate compared to standard Colchicum autumnale corm. The total alkaloid contents of all corms were 0.452% ± 0.021 to 0.982% ± 0.034. Due to high variety of Colchicum species in Iran and uses in Persian medicine and pharmacognostic and physicochemical data, these studied plants maybe candidate in the official medicinal plant list.

Biography

Dr Ali Davoodi studied in PhD of pharmacognosy from department of pharmacognosy in faculty of pharmacy of mazandaran university of medical sciences of sari, Iran. He has published more than 10 papers in reputed journals and 2 books in iranian publications. Moreover, he has worked in phytochemical analysis, secondary metabolites production and natural drug absorption enhancers.

Speaker
Ali Davoodi / Mazandaran University of Medical Sciences
Iran

Abstract

Induction of peripheral tolerance has long been considered a promising approach to the treatment of rheumatoid arthritis (RA). We aimed to evaluate the therapeutic potentialities of an altered peptide ligand (APL) derived from human heat-shock protein 60 (hHsp60) for the treatment of RA. A novel epitope of T cells located in the N terminal region of hHsp60, an autoantigen involved in the pathogenesis of autoimmune arthritis, was identified by bioinformatics tools and an APL (called CIGB-814) was designed starting from this epitope. We evaluated the therapeutic effect of this peptide in two animal models for RA (AA in Lewis rat and CIA in DBA1 mice) and in ex vivo assays using PBMC isolated from RA patients. CIGB-814 therapy reduced significantly the course of RA in both animal models and induced proliferation of regulatory T cells in ex vivo assays using PBMC from RA patients. In addition, we performed Phase I Clinical Trial with CIGB-814 in RA patients. This study was designed according three dosage levels of CIGB-814. The Schedule included 9 doses per patient in six months. Phase I Clinical Trial concluded showing safety of CIGB-814. Treatment reduced Il-17 and IFN-γ levels in patients. 17 patients achieved ACR 70, when they finished therapy. At 6 months, erosion and edema score by magnetic resonance imaging from baseline was significantly lower. These results reinforce the therapeutic possibilities of CIGB-814 as a novel candidate for treatment of RA.

Biography

Will be updated soon...

Speaker
Maria del Carmen Dominguez Horta / Center for Genetic Engineering and Biotechnology
Cuba